Abstract
We have demonstrated the feasibility of using bacteriophage ghost proteins, tritiated by metabolic labeling, as a set of standard markers for two‐dimensional gels in which the proteins are to be detected by silver staining. The results indicate that a 2.5 μg load of phage proteins yields a reproducible silver pattern of 48 spots. The spots can also be readily identified by radioautography and radiofluorography, establishing their value as a standard constellation of markers. Quantification of these patterns by computerized densitometry indicates a general agreement between detection by silver staining and detection by radiofluorography.
Original language | English (US) |
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Pages (from-to) | 87-92 |
Number of pages | 6 |
Journal | ELECTROPHORESIS |
Volume | 13 |
Issue number | 1 |
DOIs | |
State | Published - 1992 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry