THE USE OF SPECIFIC RADIOIMMUNOASSAYS TO DETERMINE ACTION SPECTRA FOR THE PHOTOLYSIS OF (6‐4) PHOTOPRODUCTS

David L. Mitchell; Barry S. Rosenstein

doi:10.1111/j.1751-1097.1987.tb07882.x

THE USE OF SPECIFIC RADIOIMMUNOASSAYS TO DETERMINE ACTION SPECTRA FOR THE PHOTOLYSIS OF (6‐4) PHOTOPRODUCTS

David L. Mitchell, Barry S. Rosenstein

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Abstract. A radioimmunoassay (RIA) was developed which specifically detects a photoproduct produced by the near‐UV photolysis of pyrimidine(6‐4)pyrimidone photoproducts. This assay was used in conjunction with a previously characterized RIA which specifically detects (6‐4) photoproducts to determine the relative efficiency of wavelengths between 265 and 435 nm for photolysing these lesions. The rate of loss of antibody‐binding sites associated with (6‐4) photoproducts correlates with the production of those associated with its photolysis product. Action spectra for both the loss of (6‐4) photoproducts and the induction of the photolysis product parallel the absorption spectrum of the (6‐4) photoproduct.

Original language	English (US)
Pages (from-to)	781-786
Number of pages	6
Journal	Photochemistry and photobiology
Volume	45
DOIs	https://doi.org/10.1111/j.1751-1097.1987.tb07882.x
State	Published - May 1987

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry

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10.1111/j.1751-1097.1987.tb07882.x

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title = "THE USE OF SPECIFIC RADIOIMMUNOASSAYS TO DETERMINE ACTION SPECTRA FOR THE PHOTOLYSIS OF (6‐4) PHOTOPRODUCTS",

abstract = "Abstract. A radioimmunoassay (RIA) was developed which specifically detects a photoproduct produced by the near‐UV photolysis of pyrimidine(6‐4)pyrimidone photoproducts. This assay was used in conjunction with a previously characterized RIA which specifically detects (6‐4) photoproducts to determine the relative efficiency of wavelengths between 265 and 435 nm for photolysing these lesions. The rate of loss of antibody‐binding sites associated with (6‐4) photoproducts correlates with the production of those associated with its photolysis product. Action spectra for both the loss of (6‐4) photoproducts and the induction of the photolysis product parallel the absorption spectrum of the (6‐4) photoproduct.",

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AU - Rosenstein, Barry S.

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N2 - Abstract. A radioimmunoassay (RIA) was developed which specifically detects a photoproduct produced by the near‐UV photolysis of pyrimidine(6‐4)pyrimidone photoproducts. This assay was used in conjunction with a previously characterized RIA which specifically detects (6‐4) photoproducts to determine the relative efficiency of wavelengths between 265 and 435 nm for photolysing these lesions. The rate of loss of antibody‐binding sites associated with (6‐4) photoproducts correlates with the production of those associated with its photolysis product. Action spectra for both the loss of (6‐4) photoproducts and the induction of the photolysis product parallel the absorption spectrum of the (6‐4) photoproduct.

AB - Abstract. A radioimmunoassay (RIA) was developed which specifically detects a photoproduct produced by the near‐UV photolysis of pyrimidine(6‐4)pyrimidone photoproducts. This assay was used in conjunction with a previously characterized RIA which specifically detects (6‐4) photoproducts to determine the relative efficiency of wavelengths between 265 and 435 nm for photolysing these lesions. The rate of loss of antibody‐binding sites associated with (6‐4) photoproducts correlates with the production of those associated with its photolysis product. Action spectra for both the loss of (6‐4) photoproducts and the induction of the photolysis product parallel the absorption spectrum of the (6‐4) photoproduct.

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THE USE OF SPECIFIC RADIOIMMUNOASSAYS TO DETERMINE ACTION SPECTRA FOR THE PHOTOLYSIS OF (6‐4) PHOTOPRODUCTS

Abstract

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