Abstract
Purpose: To evaluate the effectiveness of a neutral DOPC nanoliposome system for the delivery of siRNA to tumor cells in an obese murine cervical cancer model. Methods: In vitro silencing of E6-E7 mRNA and E7 protein using siRNAE6 or siRNAE7 was analyzed in TC-1 cells by RT-PCR and Western blot. Silencing and antitumor capacities of siRNAE7-DOPC-nanoparticles (NP) were tested in vivo in both normal and obese mice using qPCR. These NPs were administered twice a week for 15 days and tumor volume and weight were recorded. Results: Levels of in vitro E6-E7 silencing were 90% for mRNA and 60% for protein when siRNAE7 was used. On the other hand when siRNAE6 was used, the levels of silencing were 50% for E6-E7 mRNA and only 20% for protein. In vivo E7 mRNA silencing by siRNAE7-DOPC-NP was similar (60%) in both non-obese and obese mouse models. The therapeutic study showed a 65% decrease in tumor volume and a 57% reduction in tumor weight as compared to the control groups. Conclusion: There was no negative impact of obesity on the antitumor activity of siRNA-DOPC-NP in obese mice.
Original language | English (US) |
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Pages (from-to) | 1471-1479 |
Number of pages | 9 |
Journal | Journal of B.U.ON. |
Volume | 20 |
Issue number | 6 |
State | Published - Nov 1 2015 |
Keywords
- Cervical cancer
- DOPC
- HPV
- Nanoliposomes
- Obesity
- SiRNA
ASJC Scopus subject areas
- Hematology
- Oncology
- Radiology Nuclear Medicine and imaging
- Cancer Research