TY - JOUR
T1 - Tissue differences in antioxidant enzyme gene expression in response to endotoxin
AU - Ghosh, Bidyut
AU - Hanevold, Coral Dawn
AU - Dobashi, Kazushige
AU - Orak, John Kenneth
AU - Singh, Inderjit
N1 - Funding Information:
Acknowledgements -- This work was funded in part by grants from the Children's Hospital Fund, Medical University of South Carolina, D.C.I. and from National Institutes of Health N.S. 22576.
PY - 1996
Y1 - 1996
N2 - The effect of endotoxin on antioxidant gene expression and antioxidant enzyme activity in homogenates of the heart, liver, and kidney from Sprague- Dawley rats was compared by quantitation of m-RNA and enzyme activities. Alterations in the message level for Cu-Zn superoxide dismutase (SOD), Mn SOD, and catalase varied with the tissue type, length of exposure to endotoxin, and dose of endotoxin. In general, endotoxin treatment reduced Cu- Zn SOD expression in the heart and liver, but had no noticeable effect in the kidney. Mn SOD message levels were increased in the heart and kidney but decreased in the liver. Catalase expression was reduced in the kidney and increased marginally in the heart and liver. With regard to enzyme activity, endotoxin treatment reduced Cu-Zn SOD activity in the heart, liver, and kidney. Mn SOD activity showed little change in the heart, but increased in the liver and, to a lesser extent, in the kidney. Catalase activity showed little change in the heart and kidney but was decreased at 12 h in the liver. The differing responses of tissues to the oxidant stress of endotoxin exposure should be considered when evaluating the effect of endotoxin on antioxidant enzymes.
AB - The effect of endotoxin on antioxidant gene expression and antioxidant enzyme activity in homogenates of the heart, liver, and kidney from Sprague- Dawley rats was compared by quantitation of m-RNA and enzyme activities. Alterations in the message level for Cu-Zn superoxide dismutase (SOD), Mn SOD, and catalase varied with the tissue type, length of exposure to endotoxin, and dose of endotoxin. In general, endotoxin treatment reduced Cu- Zn SOD expression in the heart and liver, but had no noticeable effect in the kidney. Mn SOD message levels were increased in the heart and kidney but decreased in the liver. Catalase expression was reduced in the kidney and increased marginally in the heart and liver. With regard to enzyme activity, endotoxin treatment reduced Cu-Zn SOD activity in the heart, liver, and kidney. Mn SOD activity showed little change in the heart, but increased in the liver and, to a lesser extent, in the kidney. Catalase activity showed little change in the heart and kidney but was decreased at 12 h in the liver. The differing responses of tissues to the oxidant stress of endotoxin exposure should be considered when evaluating the effect of endotoxin on antioxidant enzymes.
KW - Catalase
KW - Endotoxin (lipopolysaccharide)
KW - Enzyme Activity
KW - Free radicals
KW - Superoxide dismutases
KW - Transcription
UR - http://www.scopus.com/inward/record.url?scp=0029841838&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029841838&partnerID=8YFLogxK
U2 - 10.1016/0891-5849(96)00048-2
DO - 10.1016/0891-5849(96)00048-2
M3 - Article
C2 - 8886805
AN - SCOPUS:0029841838
SN - 0891-5849
VL - 21
SP - 533
EP - 540
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 4
ER -