TY - JOUR
T1 - Topo IIα gene alterations correlated with survival in patients with diffuse large B-cell lymphoma
AU - Chen, Zhenwen
AU - Wang, Jinfen
AU - Zhang, Hongwei
AU - Liu, Dongmei
AU - Li, Yi
AU - Xu, Yirong
AU - Tan, Dongfeng
AU - Chen, Dong
AU - Zhao, Xia
AU - Wang, Guoping
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/3
Y1 - 2012/3
N2 - Background Topoisomerase IIα (topo IIα) protein expression has prognostic significance in many cancers. However, it is still unclear whether topo IIα protein expression and gene alterations play roles as prognostic factors in diffuse large B-cell lymphoma (DLBCL). Materials and methods We selected 102 patients with DLBCL who were homogeneously treated with CHOP chemotherapy and followed up. Using tissue microarray technology, all of the cases, consisting of 25 germinal centre B-cell-like (GCB) and 77 nongerminal centre B-cell-like (non-GCB) types, were studied. Topo IIα protein expression was detected by immunohistochemistry. Gene copy number of topo IIα was analysed by chromogenic in situ hybridization. Cox regression, chi-square test and Kaplan-Meier statistics were performed using SPSS 15·0. Results Topo IIα protein overexpression was found in 91 (91/102, 89·2%) cases, while topo IIα gene amplification was absent in all cases. Chromosome 17 deletion was identified in 3 (3/102, 2·9%) cases, diploid in 66 (66/102, 64·7%) cases and aneuploidy in 33 (33/102, 32·4%) cases. By multivariate analysis, no significant differences in progression-free survival (PFS) and overall survival (OS) were observed in patients with topo IIα protein overexpression (P>0·05), while chromosome 17 aneuploidy predicted worse PFS and OS (P<0·001). Conclusions These results suggested that chromosome 17 aneuploidy, but not topo IIα protein expression, could predict worse survival in patients with DLBCL.
AB - Background Topoisomerase IIα (topo IIα) protein expression has prognostic significance in many cancers. However, it is still unclear whether topo IIα protein expression and gene alterations play roles as prognostic factors in diffuse large B-cell lymphoma (DLBCL). Materials and methods We selected 102 patients with DLBCL who were homogeneously treated with CHOP chemotherapy and followed up. Using tissue microarray technology, all of the cases, consisting of 25 germinal centre B-cell-like (GCB) and 77 nongerminal centre B-cell-like (non-GCB) types, were studied. Topo IIα protein expression was detected by immunohistochemistry. Gene copy number of topo IIα was analysed by chromogenic in situ hybridization. Cox regression, chi-square test and Kaplan-Meier statistics were performed using SPSS 15·0. Results Topo IIα protein overexpression was found in 91 (91/102, 89·2%) cases, while topo IIα gene amplification was absent in all cases. Chromosome 17 deletion was identified in 3 (3/102, 2·9%) cases, diploid in 66 (66/102, 64·7%) cases and aneuploidy in 33 (33/102, 32·4%) cases. By multivariate analysis, no significant differences in progression-free survival (PFS) and overall survival (OS) were observed in patients with topo IIα protein overexpression (P>0·05), while chromosome 17 aneuploidy predicted worse PFS and OS (P<0·001). Conclusions These results suggested that chromosome 17 aneuploidy, but not topo IIα protein expression, could predict worse survival in patients with DLBCL.
KW - Chromosome 17 aneuploidy
KW - Diffuse large b-cell lymphoma
KW - Topoisomerase IIα
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U2 - 10.1111/j.1365-2362.2011.02585.x
DO - 10.1111/j.1365-2362.2011.02585.x
M3 - Article
C2 - 21880040
AN - SCOPUS:84856561388
SN - 0014-2972
VL - 42
SP - 310
EP - 320
JO - European Journal of Clinical Investigation
JF - European Journal of Clinical Investigation
IS - 3
ER -