TY - JOUR
T1 - Toxicity Evaluation of a Novel Magnetic Resonance Imaging Marker, CoCl2-N-Acetylcysteine, in Rats
AU - Wang, Li
AU - Gagea, Mihai
AU - Martirosyan, Karen
AU - Johansen, Mary
AU - Madden, Timothy
AU - Norberg, Lisa
AU - Culotta, Kirk S.
AU - Frank, Steven J.
N1 - Publisher Copyright:
© 2018 Li Wang et al.
PY - 2018
Y1 - 2018
N2 - C4 (cobalt dichloride-N-acetylcysteine [1% CoCl 2 :2% NAC]) is a novel magnetic resonance imaging contrast marker that facilitates visualization of implanted radioactive seeds in cancer brachytherapy. We evaluated the toxicity of C4. Rats were assigned to control (0% CoCl 2 :NAC), low-dose (0.1% CoCl 2 :2% NAC), reference-dose (C4), and high-dose (10% CoCl 2 :2% NAC) groups. Agent was injected into the left quadriceps femoris muscle of the rats. Endpoints were organ and body weights, hematology, and serum chemistry and histopathologic changes of tissues at 48 hours and 28 and 63 days after dosing. Student's t tests were used. No abnormalities in clinical signs, terminal body and organ weights, or hematologic and serum chemistry were noted, and no gross or histopathologic lesions of systemic tissue toxicity were found in any treatment group at any time point studied. At the site of injection, concentration-dependent acute responses were observed in all treatment groups at 48 hours after dosing and were recovered by 28 days. No myofiber degeneration or necrosis was observed at 28 or 63 days in any group. In conclusion, a single intramuscular dose of C4 produced no acute or chronic systemic toxicity or inflammation in rats, suggesting that C4 may be toxicologically safe for clinical use in cancer brachytherapy.
AB - C4 (cobalt dichloride-N-acetylcysteine [1% CoCl 2 :2% NAC]) is a novel magnetic resonance imaging contrast marker that facilitates visualization of implanted radioactive seeds in cancer brachytherapy. We evaluated the toxicity of C4. Rats were assigned to control (0% CoCl 2 :NAC), low-dose (0.1% CoCl 2 :2% NAC), reference-dose (C4), and high-dose (10% CoCl 2 :2% NAC) groups. Agent was injected into the left quadriceps femoris muscle of the rats. Endpoints were organ and body weights, hematology, and serum chemistry and histopathologic changes of tissues at 48 hours and 28 and 63 days after dosing. Student's t tests were used. No abnormalities in clinical signs, terminal body and organ weights, or hematologic and serum chemistry were noted, and no gross or histopathologic lesions of systemic tissue toxicity were found in any treatment group at any time point studied. At the site of injection, concentration-dependent acute responses were observed in all treatment groups at 48 hours after dosing and were recovered by 28 days. No myofiber degeneration or necrosis was observed at 28 or 63 days in any group. In conclusion, a single intramuscular dose of C4 produced no acute or chronic systemic toxicity or inflammation in rats, suggesting that C4 may be toxicologically safe for clinical use in cancer brachytherapy.
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U2 - 10.1155/2018/9173452
DO - 10.1155/2018/9173452
M3 - Article
C2 - 30631353
AN - SCOPUS:85058899607
SN - 1687-8191
VL - 2018
JO - Journal of Toxicology
JF - Journal of Toxicology
M1 - 9173452
ER -