TY - JOUR
T1 - Toxicity evaluation of mesoporous silica particles Santa Barbara No. 15 amorphous in human umbilical vein endothelial cells
T2 - influence of particle morphology
AU - Teng, Wenqi
AU - Yang, Zhaogang
AU - Wang, Suiping
AU - Xiong, Di
AU - Chen, Yifei
AU - Wu, Zhimin
N1 - Funding Information:
The Hunan Provincial Natural Science Foundation of China, Grant/Award Number: 2019JJ50584; Research Foundation of Education Bureau of Hunan Province, Grant/Award Number: 18B068; Scientific Research Fund of Hunan Provincial Education Department, Grant/Award Number: 19C1757 Funding information
Funding Information:
This work was financially supported by The Hunan Provincial Natural Science Foundation of China (No. 2020JJ4091), the Research Foundation of Education Bureau of Hunan Province (No. 18B068), Hunan Provincial Natural Science Foundation of China (Youth Program, No. 2019JJ50584), and the Scientific Research Fund of Hunan Provincial Education Department (19C1757).
Publisher Copyright:
© 2021 John Wiley & Sons, Ltd.
PY - 2021/9
Y1 - 2021/9
N2 - Morphology plays a vital role in determining the biological effects of silica nanoparticles (NPs), but its influence on the toxicity of silica NPs in endothelial cells (ECs) is still inconclusive. We synthesized five kinds of Santa Barbara 15 amorphous (SBA-15) particles with different shapes and added them to human umbilical vein endothelial cells (HUVEC). After 24 After incubation and treatment with 100 ml, more than 80% of the cells are still alive. The microgram/ml of SBA-15 indicates that SBA-15 has high biocompatibility. Fibrous SBA-15 (5) leads to the highest Si element concentration in HUVEC. No NP reduces the release of NO, and NO is an important signaling molecule in the vascular system. Only the aggregated spherical SBA-15 (3) will moderately reduce the endothelial nitric oxide synthase (eNOS) protein. Regarding transcription factors regulating eNOS, we found that all SBA-15 types significantly increased Kruppel-like factor 2 (KLF2) protein, irregular SBA-15 (1), non-aggregated spherical SBA-15 (2) and aggregation The spherical SBA-15 (3) greatly reduces KLF4 by more than 50%. Overall, our results indicate that SBA-15 with different morphologies can be internalized into HUVEC and only cause moderate cytotoxicity. All silica NPs have the smallest effect on the NO-eNOS pathway, but the irregular spherical SBA-15 reduces the eNOS modifier KLF4. The rod-shaped SBA-15 (4) seems to have higher biocompatibility because they are internalized and have negligible adverse effects on HUVEC. These results provide new evidence for the toxic effects of different forms of silica nanoparticles on HUVEC.
AB - Morphology plays a vital role in determining the biological effects of silica nanoparticles (NPs), but its influence on the toxicity of silica NPs in endothelial cells (ECs) is still inconclusive. We synthesized five kinds of Santa Barbara 15 amorphous (SBA-15) particles with different shapes and added them to human umbilical vein endothelial cells (HUVEC). After 24 After incubation and treatment with 100 ml, more than 80% of the cells are still alive. The microgram/ml of SBA-15 indicates that SBA-15 has high biocompatibility. Fibrous SBA-15 (5) leads to the highest Si element concentration in HUVEC. No NP reduces the release of NO, and NO is an important signaling molecule in the vascular system. Only the aggregated spherical SBA-15 (3) will moderately reduce the endothelial nitric oxide synthase (eNOS) protein. Regarding transcription factors regulating eNOS, we found that all SBA-15 types significantly increased Kruppel-like factor 2 (KLF2) protein, irregular SBA-15 (1), non-aggregated spherical SBA-15 (2) and aggregation The spherical SBA-15 (3) greatly reduces KLF4 by more than 50%. Overall, our results indicate that SBA-15 with different morphologies can be internalized into HUVEC and only cause moderate cytotoxicity. All silica NPs have the smallest effect on the NO-eNOS pathway, but the irregular spherical SBA-15 reduces the eNOS modifier KLF4. The rod-shaped SBA-15 (4) seems to have higher biocompatibility because they are internalized and have negligible adverse effects on HUVEC. These results provide new evidence for the toxic effects of different forms of silica nanoparticles on HUVEC.
KW - Endothelial NO Synthase (eNOS)
KW - Human Umbilical Vein Endothelial Cells (HUVEC)
KW - Kruppel-like Factor (KLF)
KW - No Signaling Pathway
KW - Santa Barbara 15 Amorphous Alloy (SBA-15)
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U2 - 10.1002/jat.4137
DO - 10.1002/jat.4137
M3 - Article
C2 - 33438233
AN - SCOPUS:85099262761
SN - 0260-437X
VL - 41
SP - 1467
EP - 1478
JO - Journal of Applied Toxicology
JF - Journal of Applied Toxicology
IS - 9
ER -