Abstract
The bcl-2 proto-oncogene suppresses apoptosis in a variety of cell types and is essential for normal renal development. PAX8 is a member of the paired box class of transcription factors and is developmentally regulated. bcl-2 and PAX8 are both expressed in the kidney during development and throughout life, demonstrating a nearly identical pattern of expression. We used transient transfection reporter assays and electrophoretic mobility shift assays to test PAX8 transcriptional regulation of bcl-2. PAX8 transcriptionally activates the bcl-2 promoter and binds to promoter sequences in vitro. These findings establish that PAX8 can transcriptionally regulate bcl-2 and suggest that suppression of apoptosis is an important event in the development and maintenance of renal tubular epithelial structures.
Original language | English (US) |
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Pages (from-to) | 3211-3215 |
Number of pages | 5 |
Journal | Anticancer research |
Volume | 17 |
Issue number | 5 A |
State | Published - Sep 1997 |
Keywords
- Apoptosis
- Pax8
- Promoter
- Transcription factor
- bcl-2-
ASJC Scopus subject areas
- Oncology
- Cancer Research