Translation of hSNM1 is mediated by an internal ribosome entry site that upregulates expression during mitosis

Xiaoshan Zhang; Christopher Richie; Randy J. Legerski

doi:10.1016/S1568-7864(02)00015-0

Translation of hSNM1 is mediated by an internal ribosome entry site that upregulates expression during mitosis

Xiaoshan Zhang, Christopher Richie, Randy J. Legerski

Genetics

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

SNM1 is involved in the repair of DNA interstrand cross-links (ICLs) in Saccharomyces cerevisiae and possibly in human cells, although relatively little is known about its biochemical function. The hSNM1 contains a long 5′ untranslated region (5′UTR) predicted to fold into a complex secondary structure, and which contains numerous short open reading frames (ORFs). We show here using bicistronic constructs that human SNM1 mRNA contains an internal ribosome entry site (IRES) that generally suppresses translation, except during mitosis where translation is upregulated. These results suggest that hSNM1 may have a mitotic function possibly involved in response to DNA interstrand cross-linking agents.

Original language	English (US)
Pages (from-to)	379-390
Number of pages	12
Journal	DNA Repair
Volume	1
Issue number	5
DOIs	https://doi.org/10.1016/S1568-7864(02)00015-0
State	Published - May 30 2002

Keywords

Cross-linking
Internal ribosome entry site (IRES)
Mitosis
Open reading frames (ORFs)

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1016/S1568-7864(02)00015-0

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title = "Translation of hSNM1 is mediated by an internal ribosome entry site that upregulates expression during mitosis",

abstract = "SNM1 is involved in the repair of DNA interstrand cross-links (ICLs) in Saccharomyces cerevisiae and possibly in human cells, although relatively little is known about its biochemical function. The hSNM1 contains a long 5′ untranslated region (5′UTR) predicted to fold into a complex secondary structure, and which contains numerous short open reading frames (ORFs). We show here using bicistronic constructs that human SNM1 mRNA contains an internal ribosome entry site (IRES) that generally suppresses translation, except during mitosis where translation is upregulated. These results suggest that hSNM1 may have a mitotic function possibly involved in response to DNA interstrand cross-linking agents.",

keywords = "Cross-linking, Internal ribosome entry site (IRES), Mitosis, Open reading frames (ORFs)",

author = "Xiaoshan Zhang and Christopher Richie and Legerski, {Randy J.}",

note = "Funding Information: This work was supported by National Institutes of Health grants CA52461, CA75160, and by a predoctoral fellowship to CR from grant CA09299. Partial funding for CR{\textquoteright}s stipend came from an American Legion Auxiliary Fellowship. We also thank Carolyn Peterson for technical advice.",

year = "2002",

month = may,

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doi = "10.1016/S1568-7864(02)00015-0",

language = "English (US)",

volume = "1",

pages = "379--390",

journal = "DNA Repair",

issn = "1568-7864",

publisher = "Elsevier",

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T1 - Translation of hSNM1 is mediated by an internal ribosome entry site that upregulates expression during mitosis

AU - Zhang, Xiaoshan

AU - Richie, Christopher

AU - Legerski, Randy J.

N1 - Funding Information: This work was supported by National Institutes of Health grants CA52461, CA75160, and by a predoctoral fellowship to CR from grant CA09299. Partial funding for CR’s stipend came from an American Legion Auxiliary Fellowship. We also thank Carolyn Peterson for technical advice.

PY - 2002/5/30

Y1 - 2002/5/30

N2 - SNM1 is involved in the repair of DNA interstrand cross-links (ICLs) in Saccharomyces cerevisiae and possibly in human cells, although relatively little is known about its biochemical function. The hSNM1 contains a long 5′ untranslated region (5′UTR) predicted to fold into a complex secondary structure, and which contains numerous short open reading frames (ORFs). We show here using bicistronic constructs that human SNM1 mRNA contains an internal ribosome entry site (IRES) that generally suppresses translation, except during mitosis where translation is upregulated. These results suggest that hSNM1 may have a mitotic function possibly involved in response to DNA interstrand cross-linking agents.

AB - SNM1 is involved in the repair of DNA interstrand cross-links (ICLs) in Saccharomyces cerevisiae and possibly in human cells, although relatively little is known about its biochemical function. The hSNM1 contains a long 5′ untranslated region (5′UTR) predicted to fold into a complex secondary structure, and which contains numerous short open reading frames (ORFs). We show here using bicistronic constructs that human SNM1 mRNA contains an internal ribosome entry site (IRES) that generally suppresses translation, except during mitosis where translation is upregulated. These results suggest that hSNM1 may have a mitotic function possibly involved in response to DNA interstrand cross-linking agents.

KW - Cross-linking

KW - Internal ribosome entry site (IRES)

KW - Mitosis

KW - Open reading frames (ORFs)

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U2 - 10.1016/S1568-7864(02)00015-0

DO - 10.1016/S1568-7864(02)00015-0

M3 - Article

C2 - 12509242

AN - SCOPUS:0037198269

SN - 1568-7864

VL - 1

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EP - 390

JO - DNA Repair

JF - DNA Repair

IS - 5

ER -

Translation of hSNM1 is mediated by an internal ribosome entry site that upregulates expression during mitosis

Abstract

Keywords

ASJC Scopus subject areas

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