TY - JOUR
T1 - Transmission of replication-defective Sindbis helper vectors encoding capsid and envelope proteins
AU - Lu, Xiongbin
AU - Silver, Jonathan
PY - 2001
Y1 - 2001
N2 - A green fluorescent protein (gfp)-encoding derivative of the replication-defective Sindbis helper vector DHBB was constructed in order to study the rate of packaging of the helper vector. The results show that despite lacking a 'packaging sequence', this vector is co-packaged about 1/300th the rate of packaging of single RNAs containing a packaging sequence. This helps explain the frequent observation of recombinant, replication-competent viruses when using first generation Sindbis packaging systems. Because of their sensitivity, gfp-encoding reporters like the one described here will be useful for measuring transfer of helper RNAs in improved alpha virus packaging systems.
AB - A green fluorescent protein (gfp)-encoding derivative of the replication-defective Sindbis helper vector DHBB was constructed in order to study the rate of packaging of the helper vector. The results show that despite lacking a 'packaging sequence', this vector is co-packaged about 1/300th the rate of packaging of single RNAs containing a packaging sequence. This helps explain the frequent observation of recombinant, replication-competent viruses when using first generation Sindbis packaging systems. Because of their sensitivity, gfp-encoding reporters like the one described here will be useful for measuring transfer of helper RNAs in improved alpha virus packaging systems.
KW - Green fluorescent protein
KW - Packaging
KW - Sindbis helper vector
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U2 - 10.1016/S0166-0934(00)00247-0
DO - 10.1016/S0166-0934(00)00247-0
M3 - Article
C2 - 11164486
AN - SCOPUS:0035154260
SN - 0166-0934
VL - 91
SP - 59
EP - 65
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -