TY - JOUR
T1 - Treatment with lenalidomide modulates T-cell immunophenotype and cytokine production in patients with chronic lymphocytic leukemia
AU - Lee, Bang Ning
AU - Gao, Hui
AU - Cohen, Evan N.
AU - Badoux, Xavier
AU - Wierda, William G.
AU - Estrov, Zeev
AU - Faderl, Stefan H.
AU - Keating, Michael J.
AU - Ferrajoli, Alessandra
AU - Reuben, James M.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Background: Lenalidomide, an immunomodulatory agent, has activity in lymphoproliferative disorders. The authors, therefore, evaluated its effects on T-cell immunophenotype and cytokine production in patients with chronic lymphocytic leukemia (CLL). Methods: To study the immunomodulatory effects of lenalidomide in CLL, the authors recruited 24 patients with untreated CLL enrolled in a phase 2 clinical trial of lenalidomide and obtained peripheral blood specimens for immunologic studies consisting of enumeration of T cells and assessing their ability to synthesize cytokines after activation through T-cell receptor (TCR). Results: After 3 cycles of therapy, patients had a significant reduction in percentage (%) and absolute lymphocyte count (ALC) and an increase in percentage of T cells, percentage of activated CD8+ T cells producing IFN-α, and percentage of regulatory T (TR) cells when compared with their respective levels before treatment. After 15 cycles of treatment, responder patients had significant reduction in percentage of lymphocytes and ALC, percentage of activated CD4+ T cells producing IL-2, IFN-α, or TNF-α, and percentage of TR cells when compared with their perspective levels after 3 cycles of treatment. Furthermore, the numbers of activated CD4+ T cells producing IL-2, IFN-α, or TNF-α, activated CD8+ T cells producing IFN-α, and TR cells normalized to the range of healthy subjects. Conclusions: Treatment with lenalidomide resulted in the normalization of functional T-cell subsets in responders, suggesting that lenalidomide may modulate cell-mediated immunity in patients with CLL.
AB - Background: Lenalidomide, an immunomodulatory agent, has activity in lymphoproliferative disorders. The authors, therefore, evaluated its effects on T-cell immunophenotype and cytokine production in patients with chronic lymphocytic leukemia (CLL). Methods: To study the immunomodulatory effects of lenalidomide in CLL, the authors recruited 24 patients with untreated CLL enrolled in a phase 2 clinical trial of lenalidomide and obtained peripheral blood specimens for immunologic studies consisting of enumeration of T cells and assessing their ability to synthesize cytokines after activation through T-cell receptor (TCR). Results: After 3 cycles of therapy, patients had a significant reduction in percentage (%) and absolute lymphocyte count (ALC) and an increase in percentage of T cells, percentage of activated CD8+ T cells producing IFN-α, and percentage of regulatory T (TR) cells when compared with their respective levels before treatment. After 15 cycles of treatment, responder patients had significant reduction in percentage of lymphocytes and ALC, percentage of activated CD4+ T cells producing IL-2, IFN-α, or TNF-α, and percentage of TR cells when compared with their perspective levels after 3 cycles of treatment. Furthermore, the numbers of activated CD4+ T cells producing IL-2, IFN-α, or TNF-α, activated CD8+ T cells producing IFN-α, and TR cells normalized to the range of healthy subjects. Conclusions: Treatment with lenalidomide resulted in the normalization of functional T-cell subsets in responders, suggesting that lenalidomide may modulate cell-mediated immunity in patients with CLL.
KW - T cells
KW - chronic lymphocytic leukemia
KW - cytokines
KW - immunomodulatory agents
UR - http://www.scopus.com/inward/record.url?scp=80051928145&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051928145&partnerID=8YFLogxK
U2 - 10.1002/cncr.25983
DO - 10.1002/cncr.25983
M3 - Article
C2 - 21858802
AN - SCOPUS:80051928145
SN - 0008-543X
VL - 117
SP - 3999
EP - 4008
JO - Cancer
JF - Cancer
IS - 17
ER -