TY - JOUR
T1 - Trisomy 7
T2 - A potential cytogenetic marker of human prostate cancer progression
AU - Bandyk, Mark G.
AU - Pisters, Louis L.
AU - Von Eshenbach, Andrew C.
AU - Chung, Leland W.K.
AU - Zhao, Lian
AU - Liang, Jan C.
AU - Troncoso, Patricia
AU - Palmer, Judy L.
PY - 1994/1
Y1 - 1994/1
N2 - We used the fluorescence in situ hybridization (FISH) method to show that chromosome 7 trisomy is associated with the progression of human prostate cancer. Thirty‐six specimens including 15 primary prostate carcinomas, 16 metastatic lesions, and 5 normal prostate tissues, as well as 2 prostate carcinoma cell lines of different tumorigenic potential, were examined for chromosome 7 aneuplaidy. Our results showed that the androgen‐unresponsive tumorigenic cell line PC‐3 exhibited a significantly higher ratio of chromosome 7 to total chromosome number than the androgen‐responsive nontumorigenic cell line LNCaP (P = 0.001). In prostate specimens, the frequency of trisomy 7 cells was significantly increased (P < 0.05) in the advanced stage tumors (C and D1) but not in the early (6) stage tumors or normal prostatic tissue. Furthermore, metastases showed a higher frequency of trisomy 7 cells than primary tumors (P = 0.005). In 2 patients with paired primary and metastatic tumors, trisomy 7 cells increased from 4‐7% in the primary tumors to 42‐45% in the metastatic tumor cells in the bone marrow. Therefore, our data suggest that trisomy 7 may be a common feature associated with local and metastatic progression and serve as a novel marker for human prostate cancer progression. Genes Chrom Concer 9:/9‐27 (1994). © 1994 Wiley‐Liss, Inc.
AB - We used the fluorescence in situ hybridization (FISH) method to show that chromosome 7 trisomy is associated with the progression of human prostate cancer. Thirty‐six specimens including 15 primary prostate carcinomas, 16 metastatic lesions, and 5 normal prostate tissues, as well as 2 prostate carcinoma cell lines of different tumorigenic potential, were examined for chromosome 7 aneuplaidy. Our results showed that the androgen‐unresponsive tumorigenic cell line PC‐3 exhibited a significantly higher ratio of chromosome 7 to total chromosome number than the androgen‐responsive nontumorigenic cell line LNCaP (P = 0.001). In prostate specimens, the frequency of trisomy 7 cells was significantly increased (P < 0.05) in the advanced stage tumors (C and D1) but not in the early (6) stage tumors or normal prostatic tissue. Furthermore, metastases showed a higher frequency of trisomy 7 cells than primary tumors (P = 0.005). In 2 patients with paired primary and metastatic tumors, trisomy 7 cells increased from 4‐7% in the primary tumors to 42‐45% in the metastatic tumor cells in the bone marrow. Therefore, our data suggest that trisomy 7 may be a common feature associated with local and metastatic progression and serve as a novel marker for human prostate cancer progression. Genes Chrom Concer 9:/9‐27 (1994). © 1994 Wiley‐Liss, Inc.
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U2 - 10.1002/gcc.2870090105
DO - 10.1002/gcc.2870090105
M3 - Article
C2 - 7507696
AN - SCOPUS:0028181127
SN - 1045-2257
VL - 9
SP - 19
EP - 27
JO - Genes, Chromosomes and Cancer
JF - Genes, Chromosomes and Cancer
IS - 1
ER -