Two-dimensional gel electrophoresis of cell proteins in childhood leukemia, with silver staining: A preliminary report

S. M. Hanash; D. G. Tubergen; R. M. Heyn; J. V. Neel; L. Sandy; G. S. Stevens; B. B. Rosenblum; R. F. Krzesicki

doi:10.1093/clinchem/28.4.1026

Two-dimensional gel electrophoresis of cell proteins in childhood leukemia, with silver staining: A preliminary report

S. M. Hanash, D. G. Tubergen, R. M. Heyn, J. V. Neel, L. Sandy, G. S. Stevens, B. B. Rosenblum, R. F. Krzesicki

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We have investigated the potential of two-dimensional electrophoresis for identifying differences in protein patterns between acute lymphocytic (ALL) and acute myelogenous leukemia (AML). We studied blood cells directly isolated from seven children with non-T, non-B ALL and five with AML. Solubilized proteins were electrophoresed with the ISO-DALT system and made visible by silver staining. More than 800 spots were routinely detected in individual gels. Patterns for multiple runs of the same sample were indistinguishable. Most of the prominent spots were present in gels from all 12 of the individuals studied. However, substantial variability in spot intensity was observed, even among patients in the same group. Five spots were consistently present in gels of all the AML patients, and either completely absent or substantially decreased in ALL gels. Three other spots were present in ALL but not AML gels. We conclude that this may be a useful tool for characterizing leukemic cells.

Original language	English (US)
Pages (from-to)	1026-1030
Number of pages	5
Journal	Unknown Journal
Volume	28
Issue number	4 II
DOIs	https://doi.org/10.1093/clinchem/28.4.1026
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1093/clinchem/28.4.1026

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title = "Two-dimensional gel electrophoresis of cell proteins in childhood leukemia, with silver staining: A preliminary report",

abstract = "We have investigated the potential of two-dimensional electrophoresis for identifying differences in protein patterns between acute lymphocytic (ALL) and acute myelogenous leukemia (AML). We studied blood cells directly isolated from seven children with non-T, non-B ALL and five with AML. Solubilized proteins were electrophoresed with the ISO-DALT system and made visible by silver staining. More than 800 spots were routinely detected in individual gels. Patterns for multiple runs of the same sample were indistinguishable. Most of the prominent spots were present in gels from all 12 of the individuals studied. However, substantial variability in spot intensity was observed, even among patients in the same group. Five spots were consistently present in gels of all the AML patients, and either completely absent or substantially decreased in ALL gels. Three other spots were present in ALL but not AML gels. We conclude that this may be a useful tool for characterizing leukemic cells.",

author = "Hanash, {S. M.} and Tubergen, {D. G.} and Heyn, {R. M.} and Neel, {J. V.} and L. Sandy and Stevens, {G. S.} and Rosenblum, {B. B.} and Krzesicki, {R. F.}",

year = "1982",

doi = "10.1093/clinchem/28.4.1026",

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T1 - Two-dimensional gel electrophoresis of cell proteins in childhood leukemia, with silver staining

T2 - A preliminary report

AU - Hanash, S. M.

AU - Tubergen, D. G.

AU - Heyn, R. M.

AU - Neel, J. V.

AU - Sandy, L.

AU - Stevens, G. S.

AU - Rosenblum, B. B.

AU - Krzesicki, R. F.

PY - 1982

Y1 - 1982

N2 - We have investigated the potential of two-dimensional electrophoresis for identifying differences in protein patterns between acute lymphocytic (ALL) and acute myelogenous leukemia (AML). We studied blood cells directly isolated from seven children with non-T, non-B ALL and five with AML. Solubilized proteins were electrophoresed with the ISO-DALT system and made visible by silver staining. More than 800 spots were routinely detected in individual gels. Patterns for multiple runs of the same sample were indistinguishable. Most of the prominent spots were present in gels from all 12 of the individuals studied. However, substantial variability in spot intensity was observed, even among patients in the same group. Five spots were consistently present in gels of all the AML patients, and either completely absent or substantially decreased in ALL gels. Three other spots were present in ALL but not AML gels. We conclude that this may be a useful tool for characterizing leukemic cells.

AB - We have investigated the potential of two-dimensional electrophoresis for identifying differences in protein patterns between acute lymphocytic (ALL) and acute myelogenous leukemia (AML). We studied blood cells directly isolated from seven children with non-T, non-B ALL and five with AML. Solubilized proteins were electrophoresed with the ISO-DALT system and made visible by silver staining. More than 800 spots were routinely detected in individual gels. Patterns for multiple runs of the same sample were indistinguishable. Most of the prominent spots were present in gels from all 12 of the individuals studied. However, substantial variability in spot intensity was observed, even among patients in the same group. Five spots were consistently present in gels of all the AML patients, and either completely absent or substantially decreased in ALL gels. Three other spots were present in ALL but not AML gels. We conclude that this may be a useful tool for characterizing leukemic cells.

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IS - 4 II

ER -

Two-dimensional gel electrophoresis of cell proteins in childhood leukemia, with silver staining: A preliminary report

Abstract

ASJC Scopus subject areas

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