TY - JOUR
T1 - Two-Dimensionality of Yeast Colony Expansion Accompanied by Pattern Formation
AU - Chen, Lin
AU - Noorbakhsh, Javad
AU - Adams, Rhys M.
AU - Samaniego-Evans, Joseph
AU - Agollah, Germaine
AU - Nevozhay, Dmitry
AU - Kuzdzal-Fick, Jennie
AU - Mehta, Pankaj
AU - Balázsi, Gábor
N1 - Publisher Copyright:
© 2014 Chen et al.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Yeasts can form multicellular patterns as they expand on agar plates, a phenotype that requires a functional copy of the FLO11 gene. Although the biochemical and molecular requirements for such patterns have been examined, the mechanisms underlying their formation are not entirely clear. Here we develop quantitative methods to accurately characterize the size, shape, and surface patterns of yeast colonies for various combinations of agar and sugar concentrations. We combine these measurements with mathematical and physical models and find that FLO11 gene constrains cells to grow near the agar surface, causing the formation of larger and more irregular colonies that undergo hierarchical wrinkling. Head-to-head competition assays on agar plates indicate that two-dimensional constraint on the expansion of FLO11 wild type (FLO11) cells confers a fitness advantage over FLO11 knockout (flo11Δ) cells on the agar surface.
AB - Yeasts can form multicellular patterns as they expand on agar plates, a phenotype that requires a functional copy of the FLO11 gene. Although the biochemical and molecular requirements for such patterns have been examined, the mechanisms underlying their formation are not entirely clear. Here we develop quantitative methods to accurately characterize the size, shape, and surface patterns of yeast colonies for various combinations of agar and sugar concentrations. We combine these measurements with mathematical and physical models and find that FLO11 gene constrains cells to grow near the agar surface, causing the formation of larger and more irregular colonies that undergo hierarchical wrinkling. Head-to-head competition assays on agar plates indicate that two-dimensional constraint on the expansion of FLO11 wild type (FLO11) cells confers a fitness advantage over FLO11 knockout (flo11Δ) cells on the agar surface.
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U2 - 10.1371/journal.pcbi.1003979
DO - 10.1371/journal.pcbi.1003979
M3 - Article
C2 - 25504059
AN - SCOPUS:84919622589
SN - 1553-734X
VL - 10
JO - PLoS computational biology
JF - PLoS computational biology
IS - 12
ER -