Abstract
The Hardy-Zuckerman 4 feline sarcoma virus (HZ4-FeSV), isolated from a feline fibrosarcoma, is a replication defective acute transforming feline retrovirus that originated by transduction of feline c-kit sequences with feline leukemia virus (FeLV). The v-kit sequences of the HZ4-FeSV, a segment of 1106 nucleotides, correspond to sequences of the cytoplasmic domain of the c-kit receptor kinase. The HZ4-FeSV is known to encode an 80-kilodalton protein with FeLV gag and kit determinants. The P80(gag-kit) protein and its associated activities from HZ4-FeSV-transformed mink cells were characterized. The P80(gag-kit) protein was found to be myristoylated, suggesting a membrane associated for this protein. In agreement with the predicted relationship with tyrosine kinases, by using the in vitro immune complex-kinase procedure, the P80(gag-kit) protein was shown to display a tyrosine-specific autophosphorylation activity. In vivo, the P80 protein was found to be phosphorylated on serine and threonine and to a lesser degree on tyrosine. In addition, potential in vivo protein substrates for tyrosine-specific phosphorylation mediated by the P80(gag-kit) kinase were identified in HZ4-FeSV-transformed cells.
Original language | English (US) |
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Pages (from-to) | 329-335 |
Number of pages | 7 |
Journal | Oncogene Research |
Volume | 5 |
Issue number | 4 |
State | Published - 1990 |
Externally published | Yes |
Keywords
- HZ4-FeSV
- tyrosine kinase
- v-kit oncogene
ASJC Scopus subject areas
- Cancer Research