Unique epitopes in RNA helicase II/Gu protein recognized by serum from a watermelon stomach patient

Mary Catharine Garcia; Juhua Zhou; Dale Henning; Frank C. Arnett; Benigno C. Valdez

doi:10.1016/S0161-5890(00)00062-6

Unique epitopes in RNA helicase II/Gu protein recognized by serum from a watermelon stomach patient

Mary Catharine Garcia, Juhua Zhou, Dale Henning, Frank C. Arnett, Benigno C. Valdez

Stem Cell Transplantation

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12 Scopus citations

Abstract

RNA helicase II/Gu (RH II/Gu) is a nucleolar antigen originally identified using an autoimmune serum from a patient with watermelon stomach. A later report showed that anti-RH II/Gu autoantibodies were also present at low frequency in connective tissue disease (CTD) patients who did not show any symptoms suggestive of a watermelon stomach lesion. In an attempt to understand the relationship between watermelon stomach, also called gastric antral vascular ectasia (GAVE), and autoimmune disorder, we identified the antigenic sites recognized by these autoantibodies. Serum Gu uniquely recognized epitopes at amino acids 646-748 of RH II/Gu and all four CTD patient sera recognized antigenic sites within amino acids 1-173. Anti-RH II/Gu serum produced by immunizing rabbit with recombinant human RH II/Gu protein bound to the same antigenic sites recognized by the CTD patient sera, but it did not recognize the serum Gu epitopes. Results are also presented showing the use of these anti-RH II/Gu antibodies in the analysis of the evolutionary conservation of RH II/Gu in human, monkey and mouse. (C) 2000 Elsevier Science Ltd.

Original language	English (US)
Pages (from-to)	351-359
Number of pages	9
Journal	Molecular Immunology
Volume	37
Issue number	7
DOIs	https://doi.org/10.1016/S0161-5890(00)00062-6
State	Published - 2000

Keywords

Connective tissue disease
Epitope
Gastric antral vascular ectasia
Nucleolus
RNA helicase II/Gu
Watermelon stomach

ASJC Scopus subject areas

Immunology
Molecular Biology

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10.1016/S0161-5890(00)00062-6

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title = "Unique epitopes in RNA helicase II/Gu protein recognized by serum from a watermelon stomach patient",

abstract = "RNA helicase II/Gu (RH II/Gu) is a nucleolar antigen originally identified using an autoimmune serum from a patient with watermelon stomach. A later report showed that anti-RH II/Gu autoantibodies were also present at low frequency in connective tissue disease (CTD) patients who did not show any symptoms suggestive of a watermelon stomach lesion. In an attempt to understand the relationship between watermelon stomach, also called gastric antral vascular ectasia (GAVE), and autoimmune disorder, we identified the antigenic sites recognized by these autoantibodies. Serum Gu uniquely recognized epitopes at amino acids 646-748 of RH II/Gu and all four CTD patient sera recognized antigenic sites within amino acids 1-173. Anti-RH II/Gu serum produced by immunizing rabbit with recombinant human RH II/Gu protein bound to the same antigenic sites recognized by the CTD patient sera, but it did not recognize the serum Gu epitopes. Results are also presented showing the use of these anti-RH II/Gu antibodies in the analysis of the evolutionary conservation of RH II/Gu in human, monkey and mouse. (C) 2000 Elsevier Science Ltd.",

keywords = "Connective tissue disease, Epitope, Gastric antral vascular ectasia, Nucleolus, RNA helicase II/Gu, Watermelon stomach",

author = "Garcia, {Mary Catharine} and Juhua Zhou and Dale Henning and Arnett, {Frank C.} and Valdez, {Benigno C.}",

note = "Funding Information: This work was supported by grant DK52341 from the National Institute of Diabetes and Digestive and Kidney Diseases to BCV, and NIAMS Specialized Center of Research (SCOR) in Scleroderma grant (1P50AR44888) and a grant from RGK Foundation to FCA. MCG was supported by the SMART program at Baylor College of Medicine through grant T35 HL07840 from the National Heart, Lung and Blood Institute.",

year = "2000",

doi = "10.1016/S0161-5890(00)00062-6",

language = "English (US)",

volume = "37",

pages = "351--359",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "7",

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T1 - Unique epitopes in RNA helicase II/Gu protein recognized by serum from a watermelon stomach patient

AU - Garcia, Mary Catharine

AU - Zhou, Juhua

AU - Henning, Dale

AU - Arnett, Frank C.

AU - Valdez, Benigno C.

N1 - Funding Information: This work was supported by grant DK52341 from the National Institute of Diabetes and Digestive and Kidney Diseases to BCV, and NIAMS Specialized Center of Research (SCOR) in Scleroderma grant (1P50AR44888) and a grant from RGK Foundation to FCA. MCG was supported by the SMART program at Baylor College of Medicine through grant T35 HL07840 from the National Heart, Lung and Blood Institute.

PY - 2000

Y1 - 2000

N2 - RNA helicase II/Gu (RH II/Gu) is a nucleolar antigen originally identified using an autoimmune serum from a patient with watermelon stomach. A later report showed that anti-RH II/Gu autoantibodies were also present at low frequency in connective tissue disease (CTD) patients who did not show any symptoms suggestive of a watermelon stomach lesion. In an attempt to understand the relationship between watermelon stomach, also called gastric antral vascular ectasia (GAVE), and autoimmune disorder, we identified the antigenic sites recognized by these autoantibodies. Serum Gu uniquely recognized epitopes at amino acids 646-748 of RH II/Gu and all four CTD patient sera recognized antigenic sites within amino acids 1-173. Anti-RH II/Gu serum produced by immunizing rabbit with recombinant human RH II/Gu protein bound to the same antigenic sites recognized by the CTD patient sera, but it did not recognize the serum Gu epitopes. Results are also presented showing the use of these anti-RH II/Gu antibodies in the analysis of the evolutionary conservation of RH II/Gu in human, monkey and mouse. (C) 2000 Elsevier Science Ltd.

AB - RNA helicase II/Gu (RH II/Gu) is a nucleolar antigen originally identified using an autoimmune serum from a patient with watermelon stomach. A later report showed that anti-RH II/Gu autoantibodies were also present at low frequency in connective tissue disease (CTD) patients who did not show any symptoms suggestive of a watermelon stomach lesion. In an attempt to understand the relationship between watermelon stomach, also called gastric antral vascular ectasia (GAVE), and autoimmune disorder, we identified the antigenic sites recognized by these autoantibodies. Serum Gu uniquely recognized epitopes at amino acids 646-748 of RH II/Gu and all four CTD patient sera recognized antigenic sites within amino acids 1-173. Anti-RH II/Gu serum produced by immunizing rabbit with recombinant human RH II/Gu protein bound to the same antigenic sites recognized by the CTD patient sera, but it did not recognize the serum Gu epitopes. Results are also presented showing the use of these anti-RH II/Gu antibodies in the analysis of the evolutionary conservation of RH II/Gu in human, monkey and mouse. (C) 2000 Elsevier Science Ltd.

KW - Connective tissue disease

KW - Epitope

KW - Gastric antral vascular ectasia

KW - Nucleolus

KW - RNA helicase II/Gu

KW - Watermelon stomach

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Unique epitopes in RNA helicase II/Gu protein recognized by serum from a watermelon stomach patient

Abstract

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