@inbook{8ea6a8936c7b4cca9c0db77ea65b24b1,
title = "Utilizing matrigel transwell invasion assay to detect and enumerate circulating tumor cells",
abstract = "Metastasis is the cause of 90% of human cancer deaths. Circulating tumor cells (CTCs) in the peripheral blood and/or lymphatic vessels are cells shed from primary tumors and considered to be precursors of metastasis. Study of CTCs allows the serial monitoring of tumor progression and may provide predictive and prognostic biomarkers in clinic. Current CTC isolation and detection technologies encounter several challenges, including: heterogeneity of CTCs, low cell viability and/or high rate of contamination post-isolation, and the inability to distinguish viable/invasive from nonviable/nonfunctional CTCs, all of which can limit in vitro and in vivo characterization of CTCs. Here, we describe a new method to detect and enumerate of CTCs based on their invasive property.",
keywords = "Circulating tumor cell, Functional CTC isolation, Invasion, Matrigel invasion assay",
author = "Xingtong Liu and Xiangwei Wu",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-7144-2_23",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "277--282",
booktitle = "Methods in Molecular Biology",
}