Validation of a dual reporter system for in vivo heat-mediated HSP70 expression in prostate tumors

Anil Shetty; Amer Najjar; Andrew M. Elliott; Adam Springer; R. Jason Stafford; Ken Diller; Juri Gelovani; John D. Hazle

doi:10.1117/12.763361

Validation of a dual reporter system for in vivo heat-mediated HSP70 expression in prostate tumors

Anil Shetty, Amer Najjar, Andrew M. Elliott, Adam Springer, R. Jason Stafford, Ken Diller, Juri Gelovani, John D. Hazle

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Minimally invasive thermal therapy is gaining ground as a new treatment modality of prostate tumors. However, further understanding of molecular events like HSP70 expression is required for treatment planning and coordination with chemotherapy and radiation. Metastatic prostate tumor (PC3 MM2) cells, transduced with reporter genes, were utilized to study the expression of HSP70 induced by normal and nanoshell-mediated heating. A correlation was noted between HSP70 expression, cellular viability and heating temperatures. This imaging paradigm can be developed into a PET-MR thermal treatment regimen, which would include dosimetry planning, real time temperature monitoring and post treatment assessment of tumor response at a cellular level.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs	https://doi.org/10.1117/12.763361
State	Published - 2008
Event	Photonic Therapeutics and Diagnostics IV - San Jose, CA, United States Duration: Jan 19 2008 → Jan 19 2008

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6842
ISSN (Print)	1605-7422

Other

Other	Photonic Therapeutics and Diagnostics IV
Country/Territory	United States
City	San Jose, CA
Period	1/19/08 → 1/19/08

Keywords

HSP70
Promoter
Prostate cancer
Reporter genes
Thermal therapy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.763361

Cite this

Shetty, A., Najjar, A., Elliott, A. M., Springer, A., Stafford, R. J., Diller, K., Gelovani, J., & Hazle, J. D. (2008). Validation of a dual reporter system for in vivo heat-mediated HSP70 expression in prostate tumors. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE Article 68420T (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6842). https://doi.org/10.1117/12.763361

Validation of a dual reporter system for in vivo heat-mediated HSP70 expression in prostate tumors. / Shetty, Anil; Najjar, Amer ; Elliott, Andrew M. et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2008. 68420T (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6842).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shetty, A, Najjar, A , Elliott, AM, Springer, A, Stafford, RJ, Diller, K, Gelovani, J & Hazle, JD 2008, Validation of a dual reporter system for in vivo heat-mediated HSP70 expression in prostate tumors. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE., 68420T, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6842, Photonic Therapeutics and Diagnostics IV, San Jose, CA, United States, 1/19/08. https://doi.org/10.1117/12.763361

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abstract = "Minimally invasive thermal therapy is gaining ground as a new treatment modality of prostate tumors. However, further understanding of molecular events like HSP70 expression is required for treatment planning and coordination with chemotherapy and radiation. Metastatic prostate tumor (PC3 MM2) cells, transduced with reporter genes, were utilized to study the expression of HSP70 induced by normal and nanoshell-mediated heating. A correlation was noted between HSP70 expression, cellular viability and heating temperatures. This imaging paradigm can be developed into a PET-MR thermal treatment regimen, which would include dosimetry planning, real time temperature monitoring and post treatment assessment of tumor response at a cellular level.",

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Validation of a dual reporter system for in vivo heat-mediated HSP70 expression in prostate tumors

Abstract

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Keywords

ASJC Scopus subject areas

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