TY - JOUR
T1 - Visualization and quantification of intraperitoneal tumors by in vivo computed tomography using negative contrast enhancement strategy in a mouse model of ovarian cancer
AU - Rampurwala, Murtuza
AU - Ravoori, Murali K.
AU - Wei, Wei
AU - Johnson, Valen E.
AU - Vikram, Raghunandan
AU - Kundra, Vikas
N1 - Funding Information:
Address all correspondence to: Vikas Kundra, MD, PhD., Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, Box 0369, 1515 Holcombe Blvd, Houston, TX 77030. E-mail: vkundra@mdanderson.org 1This work was supported by Narional Institutes of Health grants P50 CA 83639 and P30 CA-016672. Received 23 October 2008; Revised 16 February 2009; Accepted 17 February 2009 Copyright © 2009 Neoplasia Press, Inc. Open access under CC BY-NC-ND license. 1944-7124 DOI 10.1593/tlo.08199
PY - 2009/6
Y1 - 2009/6
N2 - Small animal computed tomography (CT) has poor intrinsic soft tissue contrast, limiting evaluation of intra-abdominal structures. Using standard intravascular-extracellular intravenous contrast (IE-IV) alone is theoretically limited by long acquisition times of traditional small animal scanners thatmay result in equilibration.We assessed whether a negative contrast strategy of enhancing normal tissue surrounding tumor, instead of the tumor itself, can visualize and quantify intraperitoneal (IP) cancer in a mouse model. Two and a half weeks after IP injection of Hey A8 cells, four groups of three animals each were administered serial dilutions of IV Fenestra LC (RES-IV), oral Gastroview, and IP Optiray 320. Another group of three animals was administered IV Optiray 320 (IE-IV), oral Gastroview, and IP Optiray 320 in successive combinations. Both groups were imaged by CT. Tumor and organ Hounsfield units were measured, and visualization was assessed. With increasing contrast amount, the Hounsfield unit of organs generally increased, whereas that of tumor remained essentially stable. The visualization of abdominal organs and tumor also generally increased with increasing contrastamount. Visualization of tumor and itsmargins adjacent to liver, spleen, and stomach was significantly better on administering RES-IV. However, for tumor adjacent to bladder, both IE-IV and RES-IV were equivalent. In vivo CT-derived tumor weights correlated highly with ex vivo tumor weights (r = 0.96, P < .0001, n = 15). Thus, CT using negative contrast enhancement strategy allows visualization and quantification of IP tumors. Such a strategy will also enable anatomic localization of functional signal for combination/molecular imaging.
AB - Small animal computed tomography (CT) has poor intrinsic soft tissue contrast, limiting evaluation of intra-abdominal structures. Using standard intravascular-extracellular intravenous contrast (IE-IV) alone is theoretically limited by long acquisition times of traditional small animal scanners thatmay result in equilibration.We assessed whether a negative contrast strategy of enhancing normal tissue surrounding tumor, instead of the tumor itself, can visualize and quantify intraperitoneal (IP) cancer in a mouse model. Two and a half weeks after IP injection of Hey A8 cells, four groups of three animals each were administered serial dilutions of IV Fenestra LC (RES-IV), oral Gastroview, and IP Optiray 320. Another group of three animals was administered IV Optiray 320 (IE-IV), oral Gastroview, and IP Optiray 320 in successive combinations. Both groups were imaged by CT. Tumor and organ Hounsfield units were measured, and visualization was assessed. With increasing contrast amount, the Hounsfield unit of organs generally increased, whereas that of tumor remained essentially stable. The visualization of abdominal organs and tumor also generally increased with increasing contrastamount. Visualization of tumor and itsmargins adjacent to liver, spleen, and stomach was significantly better on administering RES-IV. However, for tumor adjacent to bladder, both IE-IV and RES-IV were equivalent. In vivo CT-derived tumor weights correlated highly with ex vivo tumor weights (r = 0.96, P < .0001, n = 15). Thus, CT using negative contrast enhancement strategy allows visualization and quantification of IP tumors. Such a strategy will also enable anatomic localization of functional signal for combination/molecular imaging.
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U2 - 10.1593/tlo.08199
DO - 10.1593/tlo.08199
M3 - Article
C2 - 19412425
AN - SCOPUS:77953451148
SN - 1944-7124
VL - 2
SP - 96
EP - 106
JO - Translational Oncology
JF - Translational Oncology
IS - 2
ER -