TY - JOUR
T1 - Vitamin E inhibits renal mRNA expression of COX II, HO I, TGFβ, and osteopontin in the rat model of cyclosporine nephrotoxicity
AU - Jenkins, John K.
AU - Huang, Hong
AU - Ndebele, Kenneth
AU - Salahudeen, Abdulla K.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2001/1/27
Y1 - 2001/1/27
N2 - Background. In a rat model of cyclosporine (CsA) nephrotoxicity, vitamin E preserves renal function and reduces free radicals, vasoconstrictive thromboxanes, and tubulointerstitial fibrosis. We examined the effect of vitamin E on tubule gene expression in this model. Methods. In two of three groups, rats were treated with either CsA, or CsA plus vitamin E, whereas the control group received vehicles. We pooled purified tubules or whole kidney tissue in a novel manner to represent each treatment group, harvested RNA, and performed rigorously controlled qualitative reverse transcription-polymerase chain reaction. Results. Cyclooxygenase (COX) I mRNA was detectable in control animals, was increased by CsA, but was unchanged by vitamin E. COX II mRNA was detected in controls, was inhibited in the CsA group, and was further inhibited with vitamin E. Hemeoxygenase I and TGF-β and osteopontin mRNA were increased in the CsA-treated group and were inhibited by vitamin E. Conclusions. Our data support the involvement of free radicals, COX pathways, and pro-fibrotic genes in cyclosporine nephrotoxicity and suggest that the salutary effect of vitamin E involves the suppression of some of these genes.
AB - Background. In a rat model of cyclosporine (CsA) nephrotoxicity, vitamin E preserves renal function and reduces free radicals, vasoconstrictive thromboxanes, and tubulointerstitial fibrosis. We examined the effect of vitamin E on tubule gene expression in this model. Methods. In two of three groups, rats were treated with either CsA, or CsA plus vitamin E, whereas the control group received vehicles. We pooled purified tubules or whole kidney tissue in a novel manner to represent each treatment group, harvested RNA, and performed rigorously controlled qualitative reverse transcription-polymerase chain reaction. Results. Cyclooxygenase (COX) I mRNA was detectable in control animals, was increased by CsA, but was unchanged by vitamin E. COX II mRNA was detected in controls, was inhibited in the CsA group, and was further inhibited with vitamin E. Hemeoxygenase I and TGF-β and osteopontin mRNA were increased in the CsA-treated group and were inhibited by vitamin E. Conclusions. Our data support the involvement of free radicals, COX pathways, and pro-fibrotic genes in cyclosporine nephrotoxicity and suggest that the salutary effect of vitamin E involves the suppression of some of these genes.
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U2 - 10.1097/00007890-200101270-00028
DO - 10.1097/00007890-200101270-00028
M3 - Article
C2 - 11213082
AN - SCOPUS:0035956733
SN - 0041-1337
VL - 71
SP - 331
EP - 334
JO - Transplantation
JF - Transplantation
IS - 2
ER -