TY - JOUR
T1 - Wound microenvironment sequesters adipose-derived stem cells in a murine model of reconstructive surgery in the setting of concurrent distant malignancy
AU - Altman, Andrew M.
AU - Prantl, Lukas
AU - Muehlberg, Fabian L.
AU - Song, Yao Hua
AU - Seidensticker, Max
AU - Butler, Charles E.
AU - Alt, Eckhard U.
PY - 2011/4
Y1 - 2011/4
N2 - Background: It is unclear whether mesenchymal stem cells that are applied to regenerate wound tissues can migrate to existing tumors and enhance their growth. The authors investigated whether adipose-derived stem cells had any effect on the growth and progression of distant tumors when applied to a skin wound. Methods: The authors subcutaneously injected murine 4T1 breast cancer cells into all BALB/c-nu/nu mice. After tumor injection, mice were randomized to five groups (five mice per group) based on the means of co-introduction of green fluorescent protein-labeled adipose-derived stem cells, if any. In group 1, adipose-derived stem cells were combined and co-injected subcutaneously. In group 2, they were injected subcutaneously at a distant anatomical site. In group 3, they were injected intravenously. In group 4, they were delivered via a human acellular dermal matrix to a distant skin wound. In group 5, no adipose-derived stem cells were introduced. Results: After 2 weeks, tumor volume increased in group 1 (356.5 ± 44.4 mm), followed by group 3 (256.6 ± 47.1 mm) and then group 2 (201.6 ± 28.6 mm). In group 4, in which adipose-derived stem cells carried on acellular dermal matrix were applied to a wound distant to the primary tumor, the tumor volume was 143.8 ± 50.9 mm, which was similar to that observed in the control group (group 5; 167.8 ± 29.9 mm). Conclusions: The authors' findings suggest that the wound microenvironment can retain adipose-derived stem cells, preventing their homing and stromal contribution to a distant neoplastic focus. These findings are an important first step in establishing the feasibility and safety of utilizing adipose-derived stem cell therapy for reconstructive surgery in the setting of malignancy.
AB - Background: It is unclear whether mesenchymal stem cells that are applied to regenerate wound tissues can migrate to existing tumors and enhance their growth. The authors investigated whether adipose-derived stem cells had any effect on the growth and progression of distant tumors when applied to a skin wound. Methods: The authors subcutaneously injected murine 4T1 breast cancer cells into all BALB/c-nu/nu mice. After tumor injection, mice were randomized to five groups (five mice per group) based on the means of co-introduction of green fluorescent protein-labeled adipose-derived stem cells, if any. In group 1, adipose-derived stem cells were combined and co-injected subcutaneously. In group 2, they were injected subcutaneously at a distant anatomical site. In group 3, they were injected intravenously. In group 4, they were delivered via a human acellular dermal matrix to a distant skin wound. In group 5, no adipose-derived stem cells were introduced. Results: After 2 weeks, tumor volume increased in group 1 (356.5 ± 44.4 mm), followed by group 3 (256.6 ± 47.1 mm) and then group 2 (201.6 ± 28.6 mm). In group 4, in which adipose-derived stem cells carried on acellular dermal matrix were applied to a wound distant to the primary tumor, the tumor volume was 143.8 ± 50.9 mm, which was similar to that observed in the control group (group 5; 167.8 ± 29.9 mm). Conclusions: The authors' findings suggest that the wound microenvironment can retain adipose-derived stem cells, preventing their homing and stromal contribution to a distant neoplastic focus. These findings are an important first step in establishing the feasibility and safety of utilizing adipose-derived stem cell therapy for reconstructive surgery in the setting of malignancy.
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U2 - 10.1097/PRS.0b013e31820a6400
DO - 10.1097/PRS.0b013e31820a6400
M3 - Article
C2 - 21460655
AN - SCOPUS:79955003778
SN - 0032-1052
VL - 127
SP - 1467
EP - 1477
JO - Plastic and reconstructive surgery
JF - Plastic and reconstructive surgery
IS - 4
ER -