TY - JOUR
T1 - XPC interacts with both HHR23B and HHR23A in vivo
AU - Li, Lei
AU - Lu, Xiaoyan
AU - Peterson, Carolyn
AU - Legerski, Randy
N1 - Funding Information:
We thank Jan Hoeijmakers for generously providing us with the HHR23A and HHR23B constructs and Eva Lee for supplying us with a monoclonal anti-XPC antibody. This work was supported by Grant CA52461 from the National Cancer Institute.
PY - 1997/5/1
Y1 - 1997/5/1
N2 - XP group C protein (XPC) and a human homologue of RAD23, HHR23B, have previously been shown to copurify in a tightly associated complex. Here, we show that XPC interacts in vivo, by means of the yeast two-hybrid system, with both HHR23B and a second homologue of RAD23, HHR23A. Domain mapping studies have revealed that both RAD23 homologues interact with XPC at the same highly conserved region in the C-terminal half of the protein. XPC mutants deleted within this domain and that are highly deficient in binding both RAD23 homologues are also highly defective in complementing XPC cells in vivo. Domain mapping studies have also identified a region in the N-terminal half of HHR23B that contains the XPC interactive site. This domain is highly conserved among HHR23B, HHR23A, and RAD23.
AB - XP group C protein (XPC) and a human homologue of RAD23, HHR23B, have previously been shown to copurify in a tightly associated complex. Here, we show that XPC interacts in vivo, by means of the yeast two-hybrid system, with both HHR23B and a second homologue of RAD23, HHR23A. Domain mapping studies have revealed that both RAD23 homologues interact with XPC at the same highly conserved region in the C-terminal half of the protein. XPC mutants deleted within this domain and that are highly deficient in binding both RAD23 homologues are also highly defective in complementing XPC cells in vivo. Domain mapping studies have also identified a region in the N-terminal half of HHR23B that contains the XPC interactive site. This domain is highly conserved among HHR23B, HHR23A, and RAD23.
KW - Domain mapping
KW - Nucleotide excision repair
KW - Xeroderma pigmentosum
KW - Xeroderma pigmentosum group C protein (XPC)
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U2 - 10.1016/S0921-8777(97)00002-5
DO - 10.1016/S0921-8777(97)00002-5
M3 - Article
C2 - 9164480
AN - SCOPUS:0030991162
SN - 0921-8777
VL - 383
SP - 197
EP - 203
JO - Mutation Research - DNA Repair
JF - Mutation Research - DNA Repair
IS - 3
ER -