TY - JOUR
T1 - Yeast α2 repressor positions nucleosomes in TRP1/ARS1 chromatin
AU - Roth, S. Y.
AU - Dean, A.
AU - Simpson, R. T.
PY - 1990
Y1 - 1990
N2 - The yeast α2 repressor suppresses expression of a-mating-type-specific genes in haploid α and diploid a/α cell types. We inserted the α2-binding site into the multicopy TRP1/ARS1 yeast plasmid and examined the effects of α2 on the chromatin structure of the derivative plasmids in α cells, a cells and a/α cells. Whereas no effect on nucleosome position was observed in a cells, nucleosomes were precisely and stably positioned over sequences flanking the α2 operator in α and a/α cells. In addition, when the α2 operator was located upstream of the TRP1 gene, an extended array of positioned nucleosomes was formed in α cells and a/α cells, with formation of a nucleosome not present in a cells, and TRP1 mRNA production was substantially reduced. These data indicate that α2 causes a positioning of nucleosomes over sequences proximal to its operator in TRP1/ARS1 chromatin and suggest that changes in chromatin structure may be related to α2 repression of cell-type-specific genes.
AB - The yeast α2 repressor suppresses expression of a-mating-type-specific genes in haploid α and diploid a/α cell types. We inserted the α2-binding site into the multicopy TRP1/ARS1 yeast plasmid and examined the effects of α2 on the chromatin structure of the derivative plasmids in α cells, a cells and a/α cells. Whereas no effect on nucleosome position was observed in a cells, nucleosomes were precisely and stably positioned over sequences flanking the α2 operator in α and a/α cells. In addition, when the α2 operator was located upstream of the TRP1 gene, an extended array of positioned nucleosomes was formed in α cells and a/α cells, with formation of a nucleosome not present in a cells, and TRP1 mRNA production was substantially reduced. These data indicate that α2 causes a positioning of nucleosomes over sequences proximal to its operator in TRP1/ARS1 chromatin and suggest that changes in chromatin structure may be related to α2 repression of cell-type-specific genes.
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U2 - 10.1128/mcb.10.5.2247
DO - 10.1128/mcb.10.5.2247
M3 - Article
C2 - 2183026
AN - SCOPUS:0025267839
SN - 0270-7306
VL - 10
SP - 2247
EP - 2260
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 5
ER -