ZIC1 gene expression is controlled by DNA and histone methylation in mesenchymal proliferations

Rasoul Pourebrahim; Kim Van Dam; Marijke Bauters; Ivo De Wever; Raf Sciot; Jean Jacques Cassiman; Sabine Tejpar

doi:10.1016/j.febslet.2007.09.061

ZIC1 gene expression is controlled by DNA and histone methylation in mesenchymal proliferations

Rasoul Pourebrahim, Kim Van Dam, Marijke Bauters, Ivo De Wever, Raf Sciot, Jean Jacques Cassiman, Sabine Tejpar

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

RNA and protein analysis revealed the consistent upregulation of the neural transcription factors ZIC1 and ZIC4 in desmoid tumors and other fibroproliferative disorders. The 5′ flanking region of the ZIC1 promoter was unmethylated in desmoid tumor fibroblasts, while a hypermethylated ZIC1 promoter was found in human and mouse cell lines not expressing the gene. In addition, expressing cells showed a H3K4me2 at the ZIC1 promoter, whereas non-expressing cells showed higher levels of H3K9me2 in the same region. To our knowledge, this is the first report describing ZIC1 expression in mesenchymal proliferations and a role for DNA methylation in the control of ZIC1 expression.

Original language	English (US)
Pages (from-to)	5122-5126
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	26
DOIs	https://doi.org/10.1016/j.febslet.2007.09.061
State	Published - Oct 30 2007
Externally published	Yes

Keywords

Desmoid tumor
Fibroblasts
Methylation
ZIC

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.09.061

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@article{e16b35f50b6f40ab90b770eff026d9ba,

title = "ZIC1 gene expression is controlled by DNA and histone methylation in mesenchymal proliferations",

abstract = "RNA and protein analysis revealed the consistent upregulation of the neural transcription factors ZIC1 and ZIC4 in desmoid tumors and other fibroproliferative disorders. The 5′ flanking region of the ZIC1 promoter was unmethylated in desmoid tumor fibroblasts, while a hypermethylated ZIC1 promoter was found in human and mouse cell lines not expressing the gene. In addition, expressing cells showed a H3K4me2 at the ZIC1 promoter, whereas non-expressing cells showed higher levels of H3K9me2 in the same region. To our knowledge, this is the first report describing ZIC1 expression in mesenchymal proliferations and a role for DNA methylation in the control of ZIC1 expression.",

keywords = "Desmoid tumor, Fibroblasts, Methylation, ZIC",

author = "Rasoul Pourebrahim and {Van Dam}, Kim and Marijke Bauters and {De Wever}, Ivo and Raf Sciot and Cassiman, {Jean Jacques} and Sabine Tejpar",

note = "Funding Information: We thank Prof. Mikoshiba and Prof. Aruga for their kind donation of the ZIC1 antibody. This project was funded by the Belgian Federation against Cancer, and the Fund for Scientific Research Flanders (FWO). M. Bauters is funded by the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT-Vlaanderen). S. Tejpar is a senior clinical investigator of the Fund for Scientific Research-Flanders (Belgium; FWO). J.J. Cassiman holds the Arthur Bax and Anna Vanluffelen chair of Human genetics. ",

year = "2007",

month = oct,

day = "30",

doi = "10.1016/j.febslet.2007.09.061",

language = "English (US)",

volume = "581",

pages = "5122--5126",

journal = "FEBS Letters",

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TY - JOUR

T1 - ZIC1 gene expression is controlled by DNA and histone methylation in mesenchymal proliferations

AU - Pourebrahim, Rasoul

AU - Van Dam, Kim

AU - Bauters, Marijke

AU - De Wever, Ivo

AU - Sciot, Raf

AU - Cassiman, Jean Jacques

AU - Tejpar, Sabine

N1 - Funding Information: We thank Prof. Mikoshiba and Prof. Aruga for their kind donation of the ZIC1 antibody. This project was funded by the Belgian Federation against Cancer, and the Fund for Scientific Research Flanders (FWO). M. Bauters is funded by the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT-Vlaanderen). S. Tejpar is a senior clinical investigator of the Fund for Scientific Research-Flanders (Belgium; FWO). J.J. Cassiman holds the Arthur Bax and Anna Vanluffelen chair of Human genetics.

PY - 2007/10/30

Y1 - 2007/10/30

N2 - RNA and protein analysis revealed the consistent upregulation of the neural transcription factors ZIC1 and ZIC4 in desmoid tumors and other fibroproliferative disorders. The 5′ flanking region of the ZIC1 promoter was unmethylated in desmoid tumor fibroblasts, while a hypermethylated ZIC1 promoter was found in human and mouse cell lines not expressing the gene. In addition, expressing cells showed a H3K4me2 at the ZIC1 promoter, whereas non-expressing cells showed higher levels of H3K9me2 in the same region. To our knowledge, this is the first report describing ZIC1 expression in mesenchymal proliferations and a role for DNA methylation in the control of ZIC1 expression.

AB - RNA and protein analysis revealed the consistent upregulation of the neural transcription factors ZIC1 and ZIC4 in desmoid tumors and other fibroproliferative disorders. The 5′ flanking region of the ZIC1 promoter was unmethylated in desmoid tumor fibroblasts, while a hypermethylated ZIC1 promoter was found in human and mouse cell lines not expressing the gene. In addition, expressing cells showed a H3K4me2 at the ZIC1 promoter, whereas non-expressing cells showed higher levels of H3K9me2 in the same region. To our knowledge, this is the first report describing ZIC1 expression in mesenchymal proliferations and a role for DNA methylation in the control of ZIC1 expression.

KW - Desmoid tumor

KW - Fibroblasts

KW - Methylation

KW - ZIC

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ZIC1 gene expression is controlled by DNA and histone methylation in mesenchymal proliferations

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