TY - JOUR
T1 - Cloning, molecular analysis, and developmental expression of 3 oleosin cDNA isoforms in coconut (Cocos nucifera L.)
AU - Vargas, Andrea G.
AU - Cabanos, Cerrone S.
AU - Garcia, Roberta N.
AU - Maruyama, Nobuyuki
AU - Tecson-Mendoza, Evelyn Mae
N1 - Funding Information:
Andrea G. Vargas thanks the Philippine Council for Advanced Science and Technology, Department of Science and Technology, for the award of a scholarship grant and the Department of Biochemistry, Faculty of Pharmacy, University of Santo Tomas, for a 2-year study leave. Technical assistance by Ms Cleotilde Caldo and the support from the Institute of Plant Breeding, College of Agricultureand Food Science, University of the Philippines Los Baños are also gratefully acknowledged.
Publisher Copyright:
© 2017 The Journal of Horticultural Science & Biotechnology Trust.
PY - 2018/5/4
Y1 - 2018/5/4
N2 - Coconut stores its lipid reserves in the endosperm, specifically in oil bodies that contain proteins called oleosins which stabilise their structure. This study reports the complete cDNA sequences of 3 genes for 3 isoforms of oleosin termed CnOLE500a, CnOLE500c, and CnOLE300a with 396, 375, and 381 nucleotides, respectively. Their predicted amino acid sequences were 131, 124, and 126 residues in length, respectively, with molecular weights of 13,787, 12,982, and 12,988 Da, respectively. The complete CnOLE500a cDNA sequence had 83.1% similarity with that of CnOLE500c, while CnOLE300a cDNA had only 50.7% and 46.5% similarity with CnOLE500a and CnOLE500c, respectively. None of the 3 coconut oleosins had the 18 amino acid insertion characteristics of H-class oleosins, thus they belonged to the L-class of oleosins. However, phylogenetic analysis showed that CnOLE300a was more related to H-class oleosins from dicots. All 3 isoforms were highly expressed at all stages of coconut endosperm development. CnOLE500c exhibited 6% higher expression than CnOLE500a and 15% higher than CnOLE300a at all stages of coconut endosperm development. However, oleosin proteins were barely detectable in the solid endosperm of coconut at 5–6 months, but this increased 20-fold at 6–7 months, and increased by a further 2- and 12-fold, at 7–8 and 8–9 months, respectively.
AB - Coconut stores its lipid reserves in the endosperm, specifically in oil bodies that contain proteins called oleosins which stabilise their structure. This study reports the complete cDNA sequences of 3 genes for 3 isoforms of oleosin termed CnOLE500a, CnOLE500c, and CnOLE300a with 396, 375, and 381 nucleotides, respectively. Their predicted amino acid sequences were 131, 124, and 126 residues in length, respectively, with molecular weights of 13,787, 12,982, and 12,988 Da, respectively. The complete CnOLE500a cDNA sequence had 83.1% similarity with that of CnOLE500c, while CnOLE300a cDNA had only 50.7% and 46.5% similarity with CnOLE500a and CnOLE500c, respectively. None of the 3 coconut oleosins had the 18 amino acid insertion characteristics of H-class oleosins, thus they belonged to the L-class of oleosins. However, phylogenetic analysis showed that CnOLE300a was more related to H-class oleosins from dicots. All 3 isoforms were highly expressed at all stages of coconut endosperm development. CnOLE500c exhibited 6% higher expression than CnOLE500a and 15% higher than CnOLE300a at all stages of coconut endosperm development. However, oleosin proteins were barely detectable in the solid endosperm of coconut at 5–6 months, but this increased 20-fold at 6–7 months, and increased by a further 2- and 12-fold, at 7–8 and 8–9 months, respectively.
KW - cloning
KW - Coconut
KW - isoforms
KW - molecular analysis
KW - oleosins
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U2 - 10.1080/14620316.2017.1364145
DO - 10.1080/14620316.2017.1364145
M3 - Article
AN - SCOPUS:85028577225
SN - 1462-0316
VL - 93
SP - 255
EP - 263
JO - Journal of Horticultural Science and Biotechnology
JF - Journal of Horticultural Science and Biotechnology
IS - 3
ER -