Correlation of cell apoptosis induction with expression of human beta5 integrin on hematopoietic cells

L. Yin; X. Zhao; S. Fu; F. Garacia-Sanchez; A. B. Deisseroth

Correlation of cell apoptosis induction with expression of human beta5 integrin on hematopoietic cells

L. Yin, X. Zhao, S. Fu, F. Garacia-Sanchez, A. B. Deisseroth

Investigational Cancer Therapeutics

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVE: To investigate the function of alpha(v)beta5 integrin in hematopoietic cells. METHODS: Beta5 integrin cDNA was expressed in K562 cells through a retroviral vector system. The changes of the alpha(v)beta5 and alpha(v)beta3 integrins expression in apoptosis and differentiation induced by serum depletion were observed. RESULT: The beta5 integrin cDNA failed to express in K562 cells after the introduction of the beta5 integrin retrovirus vector pG beta5CHT. Imbalanced expression of alpha(v)beta3 and alpha(v)beta5 integrins occurred during apoptosis and differentiation induced by serum depletion. Treatment of hematopoietic cells with anti-alpha(v)beta5 monoclonal antibody inhibited the cells apoptosis. CONCLUSION: Overexpression of the alpha(v)beta5 integrin cDNA in hematopoietic cells was associated with the inhibition of cell proliferation and apoptosis.

Original language	English (US)
Pages (from-to)	13-16
Number of pages	4
Journal	Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
Volume	22
Issue number	1
State	Published - Jan 2001

ASJC Scopus subject areas

General Medicine

Cite this

@article{c9e259136b684268a195ad66faba4866,

title = "Correlation of cell apoptosis induction with expression of human beta5 integrin on hematopoietic cells",

abstract = "OBJECTIVE: To investigate the function of alpha(v)beta5 integrin in hematopoietic cells. METHODS: Beta5 integrin cDNA was expressed in K562 cells through a retroviral vector system. The changes of the alpha(v)beta5 and alpha(v)beta3 integrins expression in apoptosis and differentiation induced by serum depletion were observed. RESULT: The beta5 integrin cDNA failed to express in K562 cells after the introduction of the beta5 integrin retrovirus vector pG beta5CHT. Imbalanced expression of alpha(v)beta3 and alpha(v)beta5 integrins occurred during apoptosis and differentiation induced by serum depletion. Treatment of hematopoietic cells with anti-alpha(v)beta5 monoclonal antibody inhibited the cells apoptosis. CONCLUSION: Overexpression of the alpha(v)beta5 integrin cDNA in hematopoietic cells was associated with the inhibition of cell proliferation and apoptosis.",

author = "L. Yin and X. Zhao and S. Fu and F. Garacia-Sanchez and Deisseroth, {A. B.}",

note = "Copyright: This record is sourced from MEDLINE{\textregistered}/PubMed{\textregistered}, a database of the U.S. National Library of Medicine",

year = "2001",

month = jan,

language = "English (US)",

volume = "22",

pages = "13--16",

journal = "Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi",

issn = "0253-2727",

publisher = "Zhongguo Yixue Kexueyuan",

number = "1",

}

TY - JOUR

T1 - Correlation of cell apoptosis induction with expression of human beta5 integrin on hematopoietic cells

AU - Yin, L.

AU - Zhao, X.

AU - Fu, S.

AU - Garacia-Sanchez, F.

AU - Deisseroth, A. B.

N1 - Copyright: This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

PY - 2001/1

Y1 - 2001/1

N2 - OBJECTIVE: To investigate the function of alpha(v)beta5 integrin in hematopoietic cells. METHODS: Beta5 integrin cDNA was expressed in K562 cells through a retroviral vector system. The changes of the alpha(v)beta5 and alpha(v)beta3 integrins expression in apoptosis and differentiation induced by serum depletion were observed. RESULT: The beta5 integrin cDNA failed to express in K562 cells after the introduction of the beta5 integrin retrovirus vector pG beta5CHT. Imbalanced expression of alpha(v)beta3 and alpha(v)beta5 integrins occurred during apoptosis and differentiation induced by serum depletion. Treatment of hematopoietic cells with anti-alpha(v)beta5 monoclonal antibody inhibited the cells apoptosis. CONCLUSION: Overexpression of the alpha(v)beta5 integrin cDNA in hematopoietic cells was associated with the inhibition of cell proliferation and apoptosis.

AB - OBJECTIVE: To investigate the function of alpha(v)beta5 integrin in hematopoietic cells. METHODS: Beta5 integrin cDNA was expressed in K562 cells through a retroviral vector system. The changes of the alpha(v)beta5 and alpha(v)beta3 integrins expression in apoptosis and differentiation induced by serum depletion were observed. RESULT: The beta5 integrin cDNA failed to express in K562 cells after the introduction of the beta5 integrin retrovirus vector pG beta5CHT. Imbalanced expression of alpha(v)beta3 and alpha(v)beta5 integrins occurred during apoptosis and differentiation induced by serum depletion. Treatment of hematopoietic cells with anti-alpha(v)beta5 monoclonal antibody inhibited the cells apoptosis. CONCLUSION: Overexpression of the alpha(v)beta5 integrin cDNA in hematopoietic cells was associated with the inhibition of cell proliferation and apoptosis.

UR - http://www.scopus.com/inward/record.url?scp=77952561252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952561252&partnerID=8YFLogxK

M3 - Article

C2 - 11877043

AN - SCOPUS:77952561252

SN - 0253-2727

VL - 22

SP - 13

EP - 16

JO - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

JF - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi

IS - 1

ER -

Correlation of cell apoptosis induction with expression of human beta5 integrin on hematopoietic cells

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this