@article{02cdbb1a534848e7ad66615d0b88a361,
title = "Crystal structure of the Hhal DNA methyltransferase complexed with S-adenosyl-l-methionine",
abstract = "The first three-dimensional structure of a DNA methyltransferase is presented. The crystal structure of the DNA (cytosine-5)-methyltransferase, M.Hhal (recognition sequence: GCGC), complexed with S-adenosyl-l-methionine has been determined and refined at 2.5 {\AA} resolution. The core of the structure is dominated by sequence motifs conserved among all DNA (cytosine-5)-methyltransferases, and these are responsible for cofactor binding and methyltransferase function.",
author = "Xiaodong Cheng and Sanjay Kumar and Janos Posfai and Pflugrath, {James W.} and Roberts, {Richard J.}",
note = "Funding Information: We thank R. M. Sweet and P. Lee for valuable assistance with the data collection and for generous access to the X12-C beamline at Brookhaven National Laboratory in the Biology Department single-crystal diff raction facility of the National Synchrotron Light Source(supported by the Department of Energy of the United States). It is a pleasure to acknowledge W. Furey for his personal help with molecular averaging and S. E. V. Phillips for the original AdoMet coordinates. We thank S. Klimasauskas and D. Barford for helpful discussions of the work; K. McCloy for protein purification; C. Lin for computational assistance; and D. Barford, A. Bhagwat, R. Blumenthal, W. Jack, E. Raleigh, I. Schildkraut, and J. Horton for critical comments on the manuscript. The work was supported in part by grants from the National Institutes of Health (NIH) (GM 48127) and the National Science Foundation (DMB 8917850) to R. J. R., the National Cancer Institute (CA 45508) through Cold Spring Harbor Laboratory and NIH (GM 49245) to X. C., and an NIH fellowship (GM 15282) to S. K. The coordinates have been deposited with the Brookhaven Protein Databank.",
year = "1993",
month = jul,
day = "30",
doi = "10.1016/0092-8674(93)90421-L",
language = "English (US)",
volume = "74",
pages = "299--307",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",
}