Differential modulation of cyclin gene expression by MYC

Pidder Jansen-Dürr; Albrecht Meichle; Philipp Steiner; Michele Pagano; Kerstin Finke; Jürgen Botz; Jeannette Wessbecher; Giulio Draetta; Martin Eilers

Differential modulation of cyclin gene expression by MYC

Pidder Jansen-Dürr, Albrecht Meichle, Philipp Steiner, Michele Pagano, Kerstin Finke, Jürgen Botz, Jeannette Wessbecher, Giulio Draetta, Martin Eilers

Genomic Medicine

Research output: Contribution to journal › Article › peer-review

305 Scopus citations

Abstract

We have investigated the effects of deregulated expression of the human c-MYC protooncogene on cyclin gene expression and on the transcription factor E2F. We found that constitutive expression of MYC or activation of conditional MycER chimeras led to higher levels of cyclin A and cyclin E mRNA. Activation of cyclin A expression by MYC led to a growth factor-independent association of cyclin A and cdk2 with the transcription factor E2F and correlated with an increase in E2F transcriptional activity. In contrast, expression of the G₁ phase cyclin D1 was strongly reduced in MYC-transformed cells. In synchronized cells, repression of cyclin D1 by MYC occurred very early in the G₁ phase of the cell cycle.

Original language	English (US)
Pages (from-to)	3685-3689
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	8
State	Published - Apr 15 1993

Keywords

G phase cyclins
cell cycle

ASJC Scopus subject areas

General

Cite this

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title = "Differential modulation of cyclin gene expression by MYC",

abstract = "We have investigated the effects of deregulated expression of the human c-MYC protooncogene on cyclin gene expression and on the transcription factor E2F. We found that constitutive expression of MYC or activation of conditional MycER chimeras led to higher levels of cyclin A and cyclin E mRNA. Activation of cyclin A expression by MYC led to a growth factor-independent association of cyclin A and cdk2 with the transcription factor E2F and correlated with an increase in E2F transcriptional activity. In contrast, expression of the G1 phase cyclin D1 was strongly reduced in MYC-transformed cells. In synchronized cells, repression of cyclin D1 by MYC occurred very early in the G1 phase of the cell cycle.",

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T1 - Differential modulation of cyclin gene expression by MYC

AU - Jansen-Dürr, Pidder

AU - Meichle, Albrecht

AU - Steiner, Philipp

AU - Pagano, Michele

AU - Finke, Kerstin

AU - Botz, Jürgen

AU - Wessbecher, Jeannette

AU - Draetta, Giulio

AU - Eilers, Martin

PY - 1993/4/15

Y1 - 1993/4/15

N2 - We have investigated the effects of deregulated expression of the human c-MYC protooncogene on cyclin gene expression and on the transcription factor E2F. We found that constitutive expression of MYC or activation of conditional MycER chimeras led to higher levels of cyclin A and cyclin E mRNA. Activation of cyclin A expression by MYC led to a growth factor-independent association of cyclin A and cdk2 with the transcription factor E2F and correlated with an increase in E2F transcriptional activity. In contrast, expression of the G1 phase cyclin D1 was strongly reduced in MYC-transformed cells. In synchronized cells, repression of cyclin D1 by MYC occurred very early in the G1 phase of the cell cycle.

AB - We have investigated the effects of deregulated expression of the human c-MYC protooncogene on cyclin gene expression and on the transcription factor E2F. We found that constitutive expression of MYC or activation of conditional MycER chimeras led to higher levels of cyclin A and cyclin E mRNA. Activation of cyclin A expression by MYC led to a growth factor-independent association of cyclin A and cdk2 with the transcription factor E2F and correlated with an increase in E2F transcriptional activity. In contrast, expression of the G1 phase cyclin D1 was strongly reduced in MYC-transformed cells. In synchronized cells, repression of cyclin D1 by MYC occurred very early in the G1 phase of the cell cycle.

KW - G phase cyclins

KW - cell cycle

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Differential modulation of cyclin gene expression by MYC

Abstract

Keywords

ASJC Scopus subject areas

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