TY - JOUR
T1 - Full complexity genomic hybridization on 60-mer oligonucleotide microarrays for array comparative genomic hybridization (aCGH).
AU - Protopopov, Alexei
AU - Feng, Bin
AU - Chin, Lynda
PY - 2008
Y1 - 2008
N2 - Recurrent structural alterations, such as amplification, deletion, or translocation, are hallmark features of the cancer genome. Mapping of these DNA copy number aberrations, using approaches such as comparative genomic hybridization (CGH), has enabled the discovery of bona fide tumor suppressor genes and oncogenes. With the emergence of high-density oligo-based microarray platforms, array-based CGH has become a powerful technology that can facilitate the accurate mapping and rapid identification of novel cancer genes. Here, we describe the optimized technical protocol for comparative genomic hybridization with full-complexity genomic DNA on 60-mer oligonucleotide microarrays.
AB - Recurrent structural alterations, such as amplification, deletion, or translocation, are hallmark features of the cancer genome. Mapping of these DNA copy number aberrations, using approaches such as comparative genomic hybridization (CGH), has enabled the discovery of bona fide tumor suppressor genes and oncogenes. With the emergence of high-density oligo-based microarray platforms, array-based CGH has become a powerful technology that can facilitate the accurate mapping and rapid identification of novel cancer genes. Here, we describe the optimized technical protocol for comparative genomic hybridization with full-complexity genomic DNA on 60-mer oligonucleotide microarrays.
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M3 - Article
C2 - 18370097
SN - 1064-3745
VL - 439
SP - 87
EP - 100
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -