TY - JOUR
T1 - hSWS1•SWSAP1 is an evolutionarily conserved complex required for efficient homologous recombination repair
AU - Liu, Ting
AU - Wan, Li
AU - Wu, Yue
AU - Chen, Junjie
AU - Huang, Jun
PY - 2011/12/2
Y1 - 2011/12/2
N2 - The Shu complex in yeast plays an important role in the homologous recombination pathway, which is critical for the maintenance of genomic integrity. The identification of human SWS1 (hSWS1) as the homolog of budding yeast Shu2 implicated that the Shu complex is evolutionarily conserved. However, the human counterparts of other components in this complex have not yet been identified and characterized. Here we describe the characterization of a novel human component of this complex, SWSAP1 (hSWS1-associated protein 1)/C19orf39. We show that hSWS1 and SWSAP1 form a stable complex in vivo and in vitro. hSWS1 and SWSAP1 are mutually interdependent for their stability. We further demonstrate that the purified hSWS1•SWSAP1 complex possesses single-stranded DNA-binding activity and DNA-stimulated ATPase activity. Moreover, SWSAP1 interacts with RAD51 and RAD51 paralogs, and depletion of SWSAP1 causes defects in homologous recombination repair. Thus, our results suggest that the human Shu complex (hSWS1•SWSAP1) has an evolutionarily conserved function in homologous recombination.
AB - The Shu complex in yeast plays an important role in the homologous recombination pathway, which is critical for the maintenance of genomic integrity. The identification of human SWS1 (hSWS1) as the homolog of budding yeast Shu2 implicated that the Shu complex is evolutionarily conserved. However, the human counterparts of other components in this complex have not yet been identified and characterized. Here we describe the characterization of a novel human component of this complex, SWSAP1 (hSWS1-associated protein 1)/C19orf39. We show that hSWS1 and SWSAP1 form a stable complex in vivo and in vitro. hSWS1 and SWSAP1 are mutually interdependent for their stability. We further demonstrate that the purified hSWS1•SWSAP1 complex possesses single-stranded DNA-binding activity and DNA-stimulated ATPase activity. Moreover, SWSAP1 interacts with RAD51 and RAD51 paralogs, and depletion of SWSAP1 causes defects in homologous recombination repair. Thus, our results suggest that the human Shu complex (hSWS1•SWSAP1) has an evolutionarily conserved function in homologous recombination.
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U2 - 10.1074/jbc.M111.271080
DO - 10.1074/jbc.M111.271080
M3 - Article
C2 - 21965664
AN - SCOPUS:82355181545
SN - 0021-9258
VL - 286
SP - 41758
EP - 41766
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 48
ER -