TY - JOUR
T1 - N terminus of CtIP is critical for homologous recombination-mediated double-strand break repair
AU - Yuan, Jingsong
AU - Chen, Junjie
PY - 2009
Y1 - 2009
N2 - DNA double-strand breaks (DSBs) represent one of the most lethal types ofDNAdamage cells encounter. CtIP (also known as RBBP8) acts together with the MRN (MRE11-RAD50-NBS1) complex to promote DNA end resection and the generation of single-stranded DNA, which is critically important for homologous recombination repair. However, it is not yet clear exactly how CtIP participates in this process. Here, we demonstrate that besides the known conserved C terminus, the N terminus of CtIP protein is also required in DSB end resection and DNA damage-induced G2/M checkpoint control. We further show that both termini of CtIP can interact with the MRN complex and that the N terminus of CtIP, especially residues 22-45, binds toMRNand plays a critical role in targeting CtIP to sites of DNA breaks. Collectively, our results highlight the importance of the N terminus of CtIP in directing its localization and function in DSB repair.
AB - DNA double-strand breaks (DSBs) represent one of the most lethal types ofDNAdamage cells encounter. CtIP (also known as RBBP8) acts together with the MRN (MRE11-RAD50-NBS1) complex to promote DNA end resection and the generation of single-stranded DNA, which is critically important for homologous recombination repair. However, it is not yet clear exactly how CtIP participates in this process. Here, we demonstrate that besides the known conserved C terminus, the N terminus of CtIP protein is also required in DSB end resection and DNA damage-induced G2/M checkpoint control. We further show that both termini of CtIP can interact with the MRN complex and that the N terminus of CtIP, especially residues 22-45, binds toMRNand plays a critical role in targeting CtIP to sites of DNA breaks. Collectively, our results highlight the importance of the N terminus of CtIP in directing its localization and function in DSB repair.
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U2 - 10.1074/jbc.M109.023424
DO - 10.1074/jbc.M109.023424
M3 - Article
C2 - 19759395
AN - SCOPUS:70450242630
SN - 0021-9258
VL - 284
SP - 31746
EP - 31752
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 46
ER -