TY - JOUR
T1 - Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds
AU - Hou, Pingping
AU - Li, Yanqin
AU - Zhang, Xu
AU - Liu, Chun
AU - Guan, Jingyang
AU - Li, Honggang
AU - Zhao, Ting
AU - Ye, Junqing
AU - Yang, Weifeng
AU - Liu, Kang
AU - Ge, Jian
AU - Xu, Jun
AU - Zhang, Qiang
AU - Zhao, Yang
AU - Deng, Hongkui
PY - 2013
Y1 - 2013
N2 - Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes " are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
AB - Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes " are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications.
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U2 - 10.1126/science.1239278
DO - 10.1126/science.1239278
M3 - Article
C2 - 23868920
AN - SCOPUS:84881256653
SN - 0036-8075
VL - 341
SP - 651
EP - 654
JO - Science
JF - Science
IS - 6146
ER -