Pooled library screening with multiplexed Cpf1 library

Jintan Liu; Sanjana Srinivasan; Chieh Yuan Li; I. Lin Ho; Johnathon Rose; Mennat Allah Shaheen; Gang Wang; Wantong Yao; Angela Deem; Chris Bristow; Traver Hart; Giulio Draetta

doi:10.1038/s41467-019-10963-x

Pooled library screening with multiplexed Cpf1 library

Jintan Liu, Sanjana Srinivasan, Chieh Yuan Li, I. Lin Ho, Johnathon Rose, Mennat Allah Shaheen, Gang Wang, Wantong Yao, Angela Deem, Chris Bristow, Traver Hart, Giulio Draetta

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated that AsCpf1 can be used for functional genomics screenings and that an AsCpf1-based multiplexed library performs similarly as compared to currently available monocistronic CRISPR/Cas9 libraries, with only one vector required for each gene. We construct the smallest whole-genome CRISPR knock-out library, Mini-human, for the human genome (n = 17,032 constructs targeting 16,977 protein-coding genes), which performs favorably compared to conventional Cas9 libraries.

Original language	English (US)
Article number	3144
Journal	Nature communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-10963-x
State	Published - Dec 1 2019

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

MD Anderson CCSG core facilities

Advanced Technology Genomics Core
Bioinformatics Shared Resource
Tissue Biospecimen and Pathology Resource
Cytogenetics and Cell Authentication Core

Access to Document

10.1038/s41467-019-10963-x

Cite this

@article{e3318e16411448f4b4de56b799944798,

title = "Pooled library screening with multiplexed Cpf1 library",

abstract = "Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated that AsCpf1 can be used for functional genomics screenings and that an AsCpf1-based multiplexed library performs similarly as compared to currently available monocistronic CRISPR/Cas9 libraries, with only one vector required for each gene. We construct the smallest whole-genome CRISPR knock-out library, Mini-human, for the human genome (n = 17,032 constructs targeting 16,977 protein-coding genes), which performs favorably compared to conventional Cas9 libraries.",

author = "Jintan Liu and Sanjana Srinivasan and Li, {Chieh Yuan} and Ho, {I. Lin} and Johnathon Rose and Shaheen, {Mennat Allah} and Gang Wang and Wantong Yao and Angela Deem and Chris Bristow and Traver Hart and Giulio Draetta",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",

year = "2019",

month = dec,

day = "1",

doi = "10.1038/s41467-019-10963-x",

language = "English (US)",

volume = "10",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Pooled library screening with multiplexed Cpf1 library

AU - Liu, Jintan

AU - Srinivasan, Sanjana

AU - Li, Chieh Yuan

AU - Ho, I. Lin

AU - Rose, Johnathon

AU - Shaheen, Mennat Allah

AU - Wang, Gang

AU - Yao, Wantong

AU - Deem, Angela

AU - Bristow, Chris

AU - Hart, Traver

AU - Draetta, Giulio

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated that AsCpf1 can be used for functional genomics screenings and that an AsCpf1-based multiplexed library performs similarly as compared to currently available monocistronic CRISPR/Cas9 libraries, with only one vector required for each gene. We construct the smallest whole-genome CRISPR knock-out library, Mini-human, for the human genome (n = 17,032 constructs targeting 16,977 protein-coding genes), which performs favorably compared to conventional Cas9 libraries.

AB - Capitalizing on the inherent multiplexing capability of AsCpf1, we developed a multiplexed, high-throughput screening strategy that minimizes library size without sacrificing gene targeting efficiency. We demonstrated that AsCpf1 can be used for functional genomics screenings and that an AsCpf1-based multiplexed library performs similarly as compared to currently available monocistronic CRISPR/Cas9 libraries, with only one vector required for each gene. We construct the smallest whole-genome CRISPR knock-out library, Mini-human, for the human genome (n = 17,032 constructs targeting 16,977 protein-coding genes), which performs favorably compared to conventional Cas9 libraries.

UR - http://www.scopus.com/inward/record.url?scp=85069521111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85069521111&partnerID=8YFLogxK

U2 - 10.1038/s41467-019-10963-x

DO - 10.1038/s41467-019-10963-x

M3 - Article

C2 - 31316073

AN - SCOPUS:85069521111

SN - 2041-1723

VL - 10

JO - Nature communications

JF - Nature communications

IS - 1

M1 - 3144

ER -

Pooled library screening with multiplexed Cpf1 library

Abstract

ASJC Scopus subject areas

MD Anderson CCSG core facilities

Access to Document

Other files and links

Fingerprint

Cite this