Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

Yang Zhao; Xiaolei Yin; Han Qin; Fangfang Zhu; Haisong Liu; Weifeng Yang; Qiang Zhang; Chengang Xiang; Pingping Hou; Zhihua Song; Yanxia Liu; Jun Yong; Pengbo Zhang; Jun Cai; Meng Liu; Honggang Li; Yanqin Li; Xiuxia Qu; Kai Cui; Weiqi Zhang; Tingting Xiang; Yetao Wu; Yiding Zhao; Chun Liu; Chen Yu; Kehu Yuan; Jinning Lou; Mingxiao Ding; Hongkui Deng

doi:10.1016/j.stem.2008.10.002

Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

Yang Zhao, Xiaolei Yin, Han Qin, Fangfang Zhu, Haisong Liu, Weifeng Yang, Qiang Zhang, Chengang Xiang, Pingping Hou, Zhihua Song, Yanxia Liu, Jun Yong, Pengbo Zhang, Jun Cai, Meng Liu, Honggang Li, Yanqin Li, Xiuxia Qu, Kai Cui, Weiqi ZhangTingting Xiang, Yetao Wu, Yiding Zhao, Chun Liu, Chen Yu, Kehu Yuan, Jinning Lou, Mingxiao Ding, Hongkui Deng

Genomic Medicine

Research output: Contribution to journal › Letter › peer-review

404 Scopus citations

Abstract

Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.

Original language	English (US)
Pages (from-to)	475-479
Number of pages	5
Journal	Cell Stem Cell
Volume	3
Issue number	5
DOIs	https://doi.org/10.1016/j.stem.2008.10.002
State	Published - Nov 6 2008

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2008.10.002

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Zhao, Y, Yin, X, Qin, H, Zhu, F, Liu, H, Yang, W, Zhang, Q, Xiang, C, Hou, P, Song, Z, Liu, Y, Yong, J, Zhang, P, Cai, J, Liu, M, Li, H, Li, Y, Qu, X, Cui, K, Zhang, W, Xiang, T, Wu, Y, Zhao, Y, Liu, C, Yu, C, Yuan, K, Lou, J, Ding, M & Deng, H 2008, 'Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation', Cell Stem Cell, vol. 3, no. 5, pp. 475-479. https://doi.org/10.1016/j.stem.2008.10.002

@article{1d9e1019892c4f1d97649ea213d0683c,

title = "Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation",

abstract = "Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.",

author = "Yang Zhao and Xiaolei Yin and Han Qin and Fangfang Zhu and Haisong Liu and Weifeng Yang and Qiang Zhang and Chengang Xiang and Pingping Hou and Zhihua Song and Yanxia Liu and Jun Yong and Pengbo Zhang and Jun Cai and Meng Liu and Honggang Li and Yanqin Li and Xiuxia Qu and Kai Cui and Weiqi Zhang and Tingting Xiang and Yetao Wu and Yiding Zhao and Chun Liu and Chen Yu and Kehu Yuan and Jinning Lou and Mingxiao Ding and Hongkui Deng",

note = "Funding Information: We thank D.Y. Lu, X.C. Chi, W. Jiang, K. Xue, S.L. Lin, Y.Z. Zhang, P.H. Bai, G. Gao, and H.X. Lu for technical assistance. We thank L. Van Parijs for the pLL3.7 vector. We also thank C.Y. Wang and I. Bruce for valuable comments on this manuscript. This research was supported by a Ministry of Science and Technology grant (2006AA02A113), a Ministry of Education grant (705001), Science and Technology Plan of Beijing Municipal Government (D07050701350705), Gongjian project of Beijing Municipal Education Commission, National Basic Research Program for China (973 Program 2007CB947900), National Natural Science Foundation of China for Creative Research Groups (30421004), a Bill & Melinda Gates Foundation Grant (37871), and a 111 Project to H.D. ",

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T1 - Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

AU - Zhao, Yang

AU - Yin, Xiaolei

AU - Qin, Han

AU - Zhu, Fangfang

AU - Liu, Haisong

AU - Yang, Weifeng

AU - Zhang, Qiang

AU - Xiang, Chengang

AU - Hou, Pingping

AU - Song, Zhihua

AU - Liu, Yanxia

AU - Yong, Jun

AU - Zhang, Pengbo

AU - Cai, Jun

AU - Liu, Meng

AU - Li, Honggang

AU - Li, Yanqin

AU - Qu, Xiuxia

AU - Cui, Kai

AU - Zhang, Weiqi

AU - Xiang, Tingting

AU - Wu, Yetao

AU - Zhao, Yiding

AU - Liu, Chun

AU - Yu, Chen

AU - Yuan, Kehu

AU - Lou, Jinning

AU - Ding, Mingxiao

AU - Deng, Hongkui

N1 - Funding Information: We thank D.Y. Lu, X.C. Chi, W. Jiang, K. Xue, S.L. Lin, Y.Z. Zhang, P.H. Bai, G. Gao, and H.X. Lu for technical assistance. We thank L. Van Parijs for the pLL3.7 vector. We also thank C.Y. Wang and I. Bruce for valuable comments on this manuscript. This research was supported by a Ministry of Science and Technology grant (2006AA02A113), a Ministry of Education grant (705001), Science and Technology Plan of Beijing Municipal Government (D07050701350705), Gongjian project of Beijing Municipal Education Commission, National Basic Research Program for China (973 Program 2007CB947900), National Natural Science Foundation of China for Creative Research Groups (30421004), a Bill & Melinda Gates Foundation Grant (37871), and a 111 Project to H.D.

PY - 2008/11/6

Y1 - 2008/11/6

N2 - Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.

AB - Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.

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JO - Cell Stem Cell

JF - Cell Stem Cell

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ER -

Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

Abstract

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