Wolframin Expression Induces Novel Ion Channel Activity in Endoplasmic Reticulum Membranes and Increases Intracellular Calcium

Abdullah A Osman, Mitsuyoshi Saito, Carol Makepeace, M. Alan Permutt, Paul Schlesinger, Mike Mueckler

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Wolfram syndrome is an autosomal recessive neurodegenerative disorder associated with juvenile onset non-autoimmune diabetes mellitus and progressive optic atrophy. The disease has been attributed to mutations in the WFS1 gene, which codes for a protein predicted to possess 9-10 transmembrane segments. Little is known concerning the function of the WFS1 protein (wolframin). Endoglycosidase H digestion, immunocytochemistry, and subcellular fractionation studies all indicated that wolframin is localized to the endoplasmic reticulum in rat brain hippocampus and rat pancreatic islet β-cells, and after ectopic expression in Xenopus oocytes. Reconstitution of wolframin from oocyte membranes into planar lipid bilayers demonstrated that the protein induced a large cation-selective ion channel that was blocked by Mg2+ or Ca2+. Inositol triphosphate was capable of activating channels in the fused bilayers that were similar to channel components induced by wolframin expression. Expression of wolframin also increased cytosolic calcium levels in oocytes. Wolframin thus appears to be important in the regulation of intracellular Ca2+ homeostasis. Disruption of this function may place cells at risk to suffer inappropriate death decisions, thus accounting for the progressive β-cell loss and neuronal degeneration associated with the disease.

Original languageEnglish (US)
Pages (from-to)52755-52762
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number52
DOIs
StatePublished - Dec 26 2003

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Intracellular Membranes
Ion Channels
Endoplasmic Reticulum
Oocytes
Rats
Calcium
Membranes
Islets of Langerhans
Tungsten
Lipid bilayers
Glycoside Hydrolases
Inositol
Wolfram Syndrome
Fractionation
Medical problems
Cations
Optic Atrophy
Optics
Brain
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wolframin Expression Induces Novel Ion Channel Activity in Endoplasmic Reticulum Membranes and Increases Intracellular Calcium. / Osman, Abdullah A; Saito, Mitsuyoshi; Makepeace, Carol; Permutt, M. Alan; Schlesinger, Paul; Mueckler, Mike.

In: Journal of Biological Chemistry, Vol. 278, No. 52, 26.12.2003, p. 52755-52762.

Research output: Contribution to journalArticle

Osman, Abdullah A ; Saito, Mitsuyoshi ; Makepeace, Carol ; Permutt, M. Alan ; Schlesinger, Paul ; Mueckler, Mike. / Wolframin Expression Induces Novel Ion Channel Activity in Endoplasmic Reticulum Membranes and Increases Intracellular Calcium. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 52. pp. 52755-52762.
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